The Surface Plasmon Resonance Core consists of a Biacore 3000 instrument that uses a powerful technique called surface plasmon resonance (SPR) to monitor binding interactions between two molecules. One of the molecules is immobilized to a biosensor module. The other molecule, called the analyte, flows over the biosensor's surface and as analyte molecules bind to the immobilized molecule, optical changes result that are monitored in real-time. Computer analysis of these optical changes allows the user to determine rate constants (Ka and Kd) and affinity (KD).
The current core services include: a) selection and preparation of the biosensor chips, using investigator-supplied proteins; b) running binding assays at various analyte concentrations. c) calculating the binding constants; d) providing the raw and analyzed data to the investigator; e) and discussing the results and answering any questions them.
The Biacore 3000 instrument provides precise, sensitive, real-time results, using microgram amounts of non-radiolabeled samples. In its ligand-recovery mode, the instrument can be used as a nano-affinity column to recover binding partners from crude mixtures of potential ligands. Its robotic recovery features precisely transfer recovered samples to plates suitable for MS analyses. Because repairs to the instrument are expensive, we keep it under a comprehensive service contract and limit hands-on access to trained operators.
Lab Phone: (310) 825-7499
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