David Sigman

Edit your Faculty Profile (Deceased)
Work Titles
UCLA Member, Biological Chemistry

Contact Information:

Email Address:

sigman@ewald.mbi.ucla.edu


Work Phone Number:

310-825-8903

310-825-9417

Work Address:

Office
MBI
Los Angeles, CA 90095


Detailed Biography:

Reaction Mechanisms in Molecular Biology Reaction mechanisms in molecular biology are the principal interests of our laboratory. Presently, the design of gene specific inhibitors which are directed to the single-stranded template DNA of open complexes formed between RNA polymerase and promoters, is an important objective. New antiviral agents are one possible practical outcome of this research. We are also developing synthetic chemical proteases and nucleases capable of mapping the conformation of membrane proteins and the binding sites of DNA binding proteins. Cleavage reagents developed from DNA binding proteins will also be used as genome mapping reagents.

Publications:

A selected list of publications:

Chen, C.-h. B., Landgraf. R., Walts, A., Terwilliger, T., Schlonk, P., Sigman, D.S.   Sequence Dictated Cleavage. DNA Scission at any Preselected Sequence, Chemistry and Biology, 1998; 5: 283-292.
Gallagher, J., Zelenko, O. and Walts, A., and Sigman, D.S. (1998) "Targeted Scission of the Polypeptide Backbone of Carbonic Anhydrase by 1,10-Phenanthroline-Copper(I)   Targeted Scission of the Polypeptide Backbone of Carbonic Anhydrase by 1,10-Phenanthroline-Copper(I), Biochemistry, 1998; 37: 2096-2104.
Meijler, M., Zelenko, O, Sigman, D.S.   Chemical Mechanism of DNA Scission by 1,10-Phenanthroline-Copper. Carbonyl Oxygen of 5-Methylene Furanone Is Derived From Water, J. Am. Chem. Soc, 1997; 119: 5746-5747.
Perrin, D.M., Chen, C-h.B., Xu, Y., Pearson, L., and Sigman, David S.   Gene Specific Transcription Inhibitors. Oligonucleotides Complementary to the Template Strand of Transcription Start Sites, J. Am. Chem. Soc, 1997; 119: 5746-5747.
Pan, CQ Finkel, SE Cramton, SE Feng, JA Sigman, DS Johnson, RC   Variable structures of Fis-DNA complexes determined by flanking DNA-protein contacts Journal of molecular biology. , 1996; 264(4): 675-95.
Landgraf, R., Pan, C. Q., Sutton, C., Pearson, L., Sigman, D.S. (1996)1996   Conversion of DNA Binding Proteins Into Site Specific Cutter: Reactivity of Trp Repressor-1,10- Phenanthroline Chimeras, Protein Engineering, 1996; 9: 603-610.
Pan, C.Q., Johnson, R.C. and Sigman, D.S.   Identification of new Fis binding sites by DNA scission with Fis-1, 10-Phenanthroline-Copper (I) chimeras, Biochemistry, 1996; 35: 4326-4333.
Gallagher, J. Perrin, D.M., Chan, L., Kwong, E., Sigman, D.S.   Site-Specific Scission of Transcriptionally Active Open Complexes by 1,10-Phenanthroline-Copper. Dependence on Nucleotide Sequence of the Promoter, Chemistry and Biology, 1996; 3: 739-746.
Perrin, DM Hoang, VM Xu, Y Mazumder, A Sigman, DS   Inhibitors of Escherichia coli RNA polymerase specific for the single-stranded DNA of transcription intermediates. Tetrahedral cuprous chelates of 1,10-phenanthrolines Biochemistry. , 1996; 35(16): 5318-26.
Wu, J., Perrin, D.M., Sigman, D.S. and Kaback, H.R.   Helix packing of Lactose Premease in Eschericia coli studies by site-directed chemical cleavage, Proc. Nat?l. Acad. Sci, 1995; 92: 9186-9190.

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