Leonard Rome, Ph.D.


Work Titles
California NanoSystems Institute Associate Director, Governing Board, California NanoSystems Institute
UCLA Professor, Biological Chemistry
Education:
Degrees:
Ph.D., University of Michigan, Ann Arbor, Michigan, 1975
M.S., University of Michigan, Ann Arbor, Michigan, 1973
B.S., University of Michigan, Ann Arbor, Michigan, 1971
Honors and Awards:
1998 California State University, Northridge, CSU Chemistry Club Distinguished Lecturer
1991 UCLA School of Medicine, Excellence in Education
1983 American Cancer Society, Faculty Research Award
1981 March of Dimes, Basil O'Connor Grant
1976 NIH, Staff Fellow
1972 NIH, Predoctoral Fellow
Professional Societies:
American Association for the Advancement of Science
American Society for Neurochemistry
American Society for Biochemistry & Molecular Biology
GRAND Steering Committee (1998-2008) (Chair 2005-2006)
American Society for Cell Biology
American Association for Cancer Research
Association of American Medical Colleges (AAMC)

Contact Information:

Work Phone Number:

(310) 825-0789

Office Address:

Office
615 Charles E. Young Drive South
BSRB 350A
Los Angeles, CA 90095


Research Interest:

Engineering of a naturally occurring cellular structure

We are using molecular engineering of a naturally occurring cellular structure called a vault to develop a flexible, targetable nano-scale capsule. Vaults are abundant cellular particles of unknown function found in nearly all eukaryotes (cells containing a nucleus). Cryo-electron microscopy combined with single particle reconstruction has provided overall dimensions of the vault at 42 x 75 nanometers (a nanometer is a millionth of a meter). These measurements indicate that the vault is larger in mass and size than many viruses. The overall structure of the intact vault is like a hollow barrel with two protruding caps and an indented waist with a very thin shell surrounding an internal cavity large enough to encompass several hundred proteins. Thus, the vault particle is a nanocapsule with incredible potential for compound encapsulation, protection, and delivery. Using a well-characterized insect virus into which a cloned gene can be easily inserted, it is possible to produce large quantities of a given protein in cultured insect cells. We have collaborated with a number of groups to use this system to produce large quantities of the major vault protein (MVP). Interestingly, the protein is able to self-assemble into vault-like particles. These MVP-only vaults are somewhat irregular, often containing distorted caps. However, we have demonstrated that co-production of all three vault proteins (MVP, TEP1 and VPARP) in insect cells results in self-assembly of particles that appear identical to naturally occurring vaults.

By using molecular genetic techniques to modify the gene encoding the major vault protein, vault proteins have been produced with chemically active peptides attached to their sequence. These modified proteins are incorporated into the inside of the vault particle without altering its basic structure. We propose to produce modified vault particles in order to test the concept that vaults can be bioengineered to allow their use in a wide variety of biological applications including drug delivery, biological sensors, enzyme delivery, controlled release, and eventually as parts for nano-electrical machines.

Vault Function and Functionality

We are interested in the biogenesis and function of subcellular organelles. We have been concentrating on novel cytosolic ribonucleoprotein particles (RNPs) called vaults. Vaults were discovered in our laboratory and found to exist in most eukaryotic cells including Dictyostelium discoideum. They have an intricate shape composed of multiple arches reminiscent of cathedral vaults, hence their name. Vault size, shape and localization suggests that they may constitute a previously recognized component of the nuclear pore complex (NPC) called the nuclear pore plug. If vaults are NPC plugs, they may be involved in nucleo-cytoplasmic transport. Vaults have been shown to be upregulated in multidrug resistant (MDR) cancer cell lines and the particle may have a role in mediating drug resistance. The role of vaults in cancer may be related to the function of the vault RNA (vRNA) or one of the two high molecular weight vault proteins (TEP1 and VPARP). TEP1 is a vault protein that is shared by the nuclear RNP telomerase. In the cytoplasm as a vault component TEP1 binds the vault RNA, while in the nucleus as part of the telomerase complex TEP1 binds to the telomerase RNA. The other high molecular weight vault protein, VPARP, is a poly ADP ribose polymerase.

We are interested in elucidating the function of these unique structures and in manipulating their structure to give them new functions. We are using the baculovirus expression system to produce recombinant vaults in order to test the concept that vaults can have a broad nanosystems application as malleable nanocapsules. Toward this aim we are currently designing particles with encapsulated fluorescent probes and enzymatically active protein domains. In addition, a number of strategies are currently being considered to encapsulate chemically active small molecules into the vault particle. If successful, these vault nanocapsules can be bioengineered to allow their use in a wide variety of biological applications including drug delivery, biological sensors, enzyme delivery, controlled release, and nano-electrical machine (NEMS) applications.

Detailed Biography:

Leonard H. Rome is a cell biologist and biochemist who has served on the UCLA School of Medicine faculty since he joined the Department of Biological Chemistry in 1979. He became a full professor in 1988 and wAs Senior Associate Dean for Research in the School of Medicine from 1997 - 2012. Dr. Rome earned his B.S. in Chemistry and M.S. and Ph.D. in Biological Chemistry at the University of Michigan, Ann Arbor. He was a postdoctoral fellow at the National Institutes of Health, where he worked on lysosome biogenesis. Dr. Rome has chaired the School of Medicine Faculty Executive Committee and is actively involved in graduate and medical education. He is a recipient of the School of Medicine Award for Excellence in Education. While Senior Associate Dean for Research, he organized a strategic plan for research in the School and spearheaded campus-wide efforts in genomics, proteomics, and computational biology. His laboratory research centers on a novel cellular organelle called a "vault" which was discovered in his laboratory. Dr. Rome is presently organizing a Nanoscience Interdisciplinnary Research Team, a collaboration of disciplines including cell biologists, engineers, chemists, and structural biologists who are engineering vaults so that they can be used in drug delivery.

Publications:

A selected list of publications:

Ding Ke, Zhang Xing, Mrazek Jan, Kickhoefer Valerie A, Lai Mason, Ng Hwee L, Yang Otto O, Rome Leonard H, Zhou Z Hong   Solution Structures of Engineered Vault Particles Structure (London, England : 1993), 2018; 26(4): 619-626.e3.
Jiang Janina, Liu Guangchao, Kickhoefer Valerie A, Rome Leonard H, Li Lin-Xi, McSorley Stephen J, Kelly Kathleen A   A Protective Vaccine against Chlamydia Genital Infection Using Vault Nanoparticles without an Added Adjuvant Vaccines, 2017; 5(1): 872-881.
Yu Kang, Yau Yin Hoe, Sinha Ameya, Tan Tabitha, Kickhoefer Valerie A, Rome Leonard H, Lee Hwankyu, Shochat Susana G, Lim Sierin   Modulation of the Vault Protein-Protein Interaction for Tuning of Molecular Release Scientific reports, 2017; 7(1): 14816.
Benner Nancy L, Zang Xiaoyu, Buehler Daniel C, Kickhoefer Valerie A, Rome Michael E, Rome Leonard H, Wender Paul A   Vault Nanoparticles: Chemical Modifications for Imaging and Enhanced Delivery ACS nano, 2017; 11(1): 872-881.
Zhu Ye, Jiang Janina, Said-Sadier Najwane, Boxx Gale, Champion Cheryl, Tetlow Ashley, Kickhoefer Valerie A, Rome Leonard H, Ojcius David M, Kelly Kathleen A   Activation of the NLRP3 inflammasome by vault nanoparticles expressing a chlamydial epitope Vaccine, 2015; 33(2): 298-306.
Matsumoto Nicholas M, Buchman George W, Rome Leonard H, Maynard Heather D   Dual pH- and Temperature-Responsive Protein Nanoparticles European polymer journal, 2015; 69(11): 532-539.
Wang Meng, Abad Danny, Kickhoefer Valerie A, Rome Leonard H, Mahendra Shaily   Vault Nanoparticles Packaged with Enzymes as an Efficient Pollutant Biodegradation Technology ACS nano, 2015; 9(11): 10931-40.
Buehler Daniel C, Marsden Matthew D, Shen Sean, Toso Daniel B, Wu Xiaomeng, Loo Joseph A, Zhou Z Hong, Kickhoefer Valerie A, Wender Paul A, Zack Jerome A, Rome Leonard H   Bioengineered vaults: self-assembling protein shell-lipophilic core nanoparticles for drug delivery ACS nano, 2014; 8(8): 7723-32.
Mrazek Jan, Toso Daniel, Ryazantsev Sergey, Zhang Xing, Zhou Z Hong, Fernandez Beatriz Campo, Kickhoefer Valerie A, Rome Leonard H   Polyribosomes are molecular 3D nanoprinters that orchestrate the assembly of vault particles ACS nano, 2014; 8(11): 11552-9.
Sharma Sherven, Zhu Li, Srivastava Minu K, Harris-White Marni, Huang Min, Lee Jay M, Rosen Fran, Lee Gina, Wang Gerald, Kickhoefer Valerie, Rome Leonard H, Baratelli Felicita, St John Maie, Reckamp Karen, Chul-Yang Seok, Hillinger Sven, Strieter Robert, Dubinett Steven   CCL21 Chemokine Therapy for Lung Cancer International trends in immunity, 2013; 1(1): 10-15.
Matsumoto Nicholas M, Prabhakaran Panchami, Rome Leonard H, Maynard Heather D   Smart vaults: thermally-responsive protein nanocapsules ACS nano, 2013; 7(1): 867-74.
Rome Leonard H, Kickhoefer Valerie A   Development of the Vault Particle as a Platform Technology ACS nano, 2012; 7(1): .
Kar Upendra K, Jiang Janina, Champion Cheryl I, Salehi Sahar, Srivastava Minu, Sharma Sherven, Rabizadeh Shahrooz, Niazi Kayvan, Kickhoefer Valerie, Rome Leonard H, Kelly Kathleen A   Vault nanocapsules as adjuvants favor cell-mediated over antibody-mediated immune responses following immunization of mice PloS one, 2012; 7(7): e38553.
Yang Jian, Srinivasan Aswin, Sun Yang, Mrazek Jan, Shu Zhanyong, Kickhoefer Valerie A, Rome Leonard H   Vault nanoparticles engineered with the protein transduction domain, TAT48, enhances cellular uptake Integrative biology : quantitative biosciences from nano to macro, 2012; .
Upendra K. Kar, Minu K. Srivastava, Åsa Andersson, Felicita Baratelli, Min Huang, Valerie A. Kickhoefer, Steven M. Dubinett, Leonard H. Rome, Sherven Sharma   Novel CCL21-Vault Nanocapsule Intratumoral Delivery Inhibits Lung Cancer Growth PLoS One, 2011; 6(5): e18758.
Han Muri, Kickhoefer Valerie A, Nemerow Glen R, Rome Leonard H   Targeted vault nanoparticles engineered with an endosomolytic peptide deliver biomolecules to the cytoplasm ACS nano, 2011; 5(8): 6128-37.
Daniel C. Buehler, Daniel B. Toso, Valerie A. Kickhoefer, Z. Hong Zhou, Leonard H. Rome   Vaults Engineered for Hydrophobic Drug Delivery Small, 2011; Epub ahead of print: .
Buehler Daniel C, Toso Daniel B, Kickhoefer Valerie A, Zhou Z Hong, Rome Leonard H   Vaults engineered for hydrophobic drug delivery Small (Weinheim an der Bergstrasse, Germany), 2011; 7(10): 1432-9.
Xia Y, Ramgopal Y, Li H, Shang L, Srinivas P, Kickhoefer VA, Rome LH, Preiser PR, Boey F, Zhang H, Venkatraman SS   Immobilization of Recombinant Vault Nanoparticles on Solid Substrates ACS Nano, 2010; 4(3): 1417-24.
Xia Yun, Ramgopal Yamini, Li Hai, Shang Lei, Srinivas Parisa, Kickhoefer Valerie A, Rome Leonard H, Preiser Peter R, Boey Freddy, Zhang Hua, Venkatraman Subbu S   Immobilization of recombinant vault nanoparticles on solid substrates ACS nano, 2010; 4(3): 1417-24.
Yang J, Kickhoefer VA, Ng BC, Gopal A, Bentolila LA, John S, Tolbert SH, Rome LH   Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange ACS Nano, 2010; 4(12): 7229-40.
Yang Jian, Kickhoefer Valerie A, Ng Benny C, Gopal Ajaykumar, Bentolila Laurent A, John Scott, Tolbert Sarah H, Rome Leonard H   Vaults are dynamically unconstrained cytoplasmic nanoparticles capable of half vault exchange ACS nano, 2010; 4(12): 7229-40.
Champion Cheryl I, Kickhoefer Valerie A, Liu Guangchao, Moniz Raymond J, Freed Amanda S, Bergmann Liisa L, Vaccari Dana, Raval-Fernandes Sujna, Chan Ann M, Rome Leonard H, Kelly Kathleen A   A vault nanoparticle vaccine induces protective mucosal immunity PloS one, 2009; 4(4): e5409.
Kickhoefer, V. A., Han, M., Raval-Fernandes, S., Poderycki, M. J., Moniz, R. J., Vaccari, D., Silvestry, M., Stewart, P. L., Kelly, K. A., Rome, L. H.   Targeting vault nanoparticles to specific cell surface receptors ACS Nano, 2009; 3(1): 27-36.
Kickhoefer Valerie A, Han Muri, Raval-Fernandes Sujna, Poderycki Michael J, Moniz Raymond J, Vaccari Dana, Silvestry Mariena, Stewart Phoebe L, Kelly Kathleen A, Rome Leonard H   Targeting vault nanoparticles to specific cell surface receptors ACS nano, 2009; 3(1): 27-36.
Goldsmith LE, Pupols M, Kickhoefer VA, Rome LH, Monbouquette HG   Utilization of a protein "shuttle" to load vault nanocapsules with gold probes and proteins ACS Nano, 2009; 3(10): 3175-83.
Goldsmith Lisa E, Pupols Melody, Kickhoefer Valerie A, Rome Leonard H, Monbouquette Harold G   Utilization of a protein "shuttle" to load vault nanocapsules with gold probes and proteins ACS nano, 2009; 3(10): 3175-83.
Lai CY, Wiethoff CM, Kickhoefer VA, Rome LH, Nemerow GR   Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer ACS Nano, 2009; 3(3): 691-9.
Lai Cheng-Yu, Wiethoff Chris M, Kickhoefer Valerie A, Rome Leonard H, Nemerow Glen R   Vault nanoparticles containing an adenovirus-derived membrane lytic protein facilitate toxin and gene transfer ACS nano, 2009; 3(3): 691-9.
Ng, B.C., Yu, M., Gopal, A., Rome, L.H., Monbouquette, H.G., Tolbert, S.H.   Encapsulation of semiconducting polymers in vault protein cages Nano Letters, 2008; 8(10): 3503-9.
Xia, T., Rome, L.H., Nel, A.   Nanobiology: Particles slip cell security Nature Materials, 2008; 7(7): 519-20.
Yu, M., Ng, B.C., Rome, L.H., Tolbert, S.H., Monbouquette, H.G.   Reversible pH lability of cross-linked vault nanocapsules Nano Letters, 2008; 8(10): 3510-5.
Esfandiary, R., Kickhoefer, V.A., Rome, L.H., Joshi, S.B., Middaugh, C.R.   Structural stability of vault particles Journal of Pharmaceutical Sciences, 2008; 98(4): 1376-86.
Anderson, D.H., Kickhoefer, V.A., Sievers, S.A., Rome, L.H., Eisenberg, D.   Draft crystal structure of the vault shell at 9-A resolution PLoS biology, 2007; 5(11): e318.
Kaddis, C.S., Lomeli, S.H., Yin, S., Berhane, B., Apostol, M.I., Kickhoefer, V.A., Rome, L.H., Loo, J.A.   Sizing large proteins and protein complexes by electrospray ionization mass spectrometry and ion mobility Journal of the American Society for Mass Spectrometry, 2007; 18(7): 1206-16.
Goldsmith, L.E., Yu, M., Rome, L.H., Monbouquette, H.G.   Vault nanocapsule dissociation into halves triggered at low pH Biochemistry, 2007; 46(10): 2865-75.
Slesina, M., Inman, E.M., Moore, A.E., Goldhaber, J.I., Rome, L.H., Volknandt, W.   Movement of vault particles visualized by GFP-tagged major vault protein Cell and Tissue Research, 2006; 324(3): 403-10.
Poderycki, M.J., Kickhoefer, V.A., Kaddis, C.S., Raval-Fernandes, S., Johansson, E., Zink, J.I., Loo, J.A., Rome, L.H.   The vault exterior shell is a dynamic structure that allows incorporation of vault-associated proteins into its interior Biochemistry, 2006; 45(39): 12184-93.
Kickhoefer, V.A., Garcia, Y., Mikyas, Y., Johansson, E., Zhou, J.C., Raval-Fernandes, S., Minoofar, P., Zink, J.I., Dunn, B., Stewart, P.L., Rome, L.H.   Engineering of vault nanocapsules with enzymatic and fluorescent properties Proceedings of the National Academy of Sciences of the United States of America, 2005; 102(12): 4348-52.
Raval-Fernandes, S., Kickhoefer, V.A., Kitchen, C., Rome, L.H.   Increased susceptibility of vault poly(ADP-ribose) polymerase-deficient mice to carcinogen-induced tumorigenesis Cancer Research, 2005; 65(19): 8846-52.
Slesina, M., Inman, E.M., Rome, L.H., Volknandt, W.   Nuclear localization of the major vault protein in U373 cells Cell and Tissue Research, 2005; 321(1): 97-104.
Poderycki, M.J., Rome, L.H., Harrington, L., Kickhoefer, V.A.   The p80 homology region of TEP1 is sufficient for its association with the telomerase and vault RNAs, and the vault particle Nucleic Acids Research, 2005; 33(3): 893-902.
Emre, N., Raval-Fernandes, S., Kickhoefer, V.A., Rome, L.H.   Analysis of MVP and VPARP promoters indicates a role for chromatin remodeling in the regulation of MVP Biochimica et Biophysica Acta, 2004; 1678(1): 33-46.
Mikyas, Y., Makabi, M., Raval-Fernandes, S., Harrington, L., Kickhoefer, V.A., Rome, L.H., Stewart, P.L.   Cryoelectron microscopy imaging of recombinant and tissue derived vaults: localization of the MVP N termini and VPARP Journal of Molecular Biology, 2004; 344(1): 91-105.
Liu, Y., Snow, B.E., Kickhoefer, V.A., Erdmann, N., Zhou, W., Wakeham, A., Gomez, M., Rome, L.H., Harrington, L.   Vault poly(ADP-ribose) polymerase is associated with mammalian telomerase and is dispensable for telomerase function and vault structure in vivo Molecular and Cellular Biology, 2004; 24(12): 5314-23.
Kickhoefer, V.A., Emre, N., Stephen, A.G., Poderycki, M.J., Rome, L.H.   Identification of conserved vault RNA expression elements and a non-expressed mouse vault RNA gene Gene, 2003; 309(2): 65-70.
Hu, Y., Stephen, A.G., Cao, J., Tanzer, L.R., Slapak, C.A., Harrison, S.D., Devanarayan, V., Dantzig, A.H., Starling, J.J., Rome, L.H., Moore, R.E.   A very early induction of major vault protein accompanied by increased drug resistance in U-937 cells International journal of cancer, 2002; 97(2): 149-56.
Kickhoefer, V.A., Poderycki, M.J., Chan, E.K.L., Rome, L.H.   The La RNA-binding protein interacts with the vault RNA and is a vault-associated protein The Journal of Biological Chemistry, 2002; 277(43): 41282-6.
Stephen, A. G., Raval-Fernandes, S., Huynh, T., Torres, M., Kickhoefer, V. A., Rome, L. H.   Assembly of vault-like particles in insect cells expressing only the major vault protein J Biol Chem, 2001; 276(26): 23217-20.
Stephen A G, Raval-Fernandes S, Huynh T, Torres M, Kickhoefer V A, Rome L H   Assembly of vault-like particles in insect cells expressing only the major vault protein The Journal of biological chemistry, 2001; 276(26): 23217-20.
Kickhoefer, V. A., Liu, Y., Kong, L. B., Snow, B. E., Stewart, P. L., Harrington, L., Rome, L. H.   The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle J Cell Biol, 2001; 152(1): 157-64.
Kickhoefer V A, Liu Y, Kong L B, Snow B E, Stewart P L, Harrington L, Rome L H   The Telomerase/vault-associated protein TEP1 is required for vault RNA stability and its association with the vault particle The Journal of cell biology, 2001; 152(1): 157-64.
Siva, A. C., Raval-Fernandes, S., Stephen, A. G., LaFemina, M. J., Scheper, R. J., Kickhoefer, V. A., Rome, L. H.   Up-regulation of vaults may be necessary but not sufficient for multidrug resistance Int J Cancer, 2001; 92(2): 195-202.
Siva A C, Raval-Fernandes S, Stephen A G, LaFemina M J, Scheper R J, Kickhoefer V A, Rome L H   Up-regulation of vaults may be necessary but not sufficient for multidrug resistance International journal of cancer. Journal international du cancer, 2001; 92(2): 195-202.
Marbois, B. N., Faull, K. F., Fluharty, A. L., Raval-Fernandes, S., Rome, L. H.   Analysis of sulfatide from rat cerebellum and multiple sclerosis white matter by negative ion electrospray mass spectrometry Biochim Biophys Acta, 2000; 1484(1): 59-70.
Marbois B N, Faull K F, Fluharty A L, Raval-Fernandes S, Rome L H   Analysis of sulfatide from rat cerebellum and multiple sclerosis white matter by negative ion electrospray mass spectrometry Biochimica et biophysica acta, 2000; 1484(1): 59-70.
Kong, L. B., Siva, A. C., Kickhoefer, V. A., Rome, L. H., Stewart, P. L.   RNA location and modeling of a WD40 repeat domain within the vault , 2000; 6(6): 890-900.
Kong L B, Siva A C, Kickhoefer V A, Rome L H, Stewart P L   RNA location and modeling of a WD40 repeat domain within the vault RNA (New York, N.Y.), 2000; 6(6): 890-900.
Schroeijers A B, Siva A C, Scheffer G L, de Jong M C, Bolick S C, Dukers D F, Slootstra J W, Meloen R H, Wiemer E, Kickhoefer V A, Rome L H, Scheper R J   The Mr 193,000 vault protein is up-regulated in multidrug-resistant cancer cell lines Cancer research, 2000; 60(4): 1104-10.
Raval-Fernandes S, Kickhoefer V A, Rome L H   Cloning of a cDNA encoding a sequence-specific single-stranded-DNA-binding protein from Rattus norvegicus Gene, 1999; 237(1): 201-7.
Kong L B, Siva A C, Rome L H, Stewart P L   Structure of the vault, a ubiquitous celular component Structure (London, England : 1993), 1999; 7(4): 371-9.
Kickhoefer V A, Siva A C, Kedersha N L, Inman E M, Ruland C, Streuli M, Rome L H   The 193-kD vault protein, VPARP, is a novel poly(ADP-ribose) polymerase The Journal of cell biology, 1999; 146(5): 917-28.
Kickhoefer V A, Stephen A G, Harrington L, Robinson M O, Rome L H   Vaults and telomerase share a common subunit, TEP1 The Journal of biological chemistry, 1999; 274(46): 32712-7.
Raval-Fernandes S, Rome L H   Role of axonal components during myelination Microscopy research and technique, 1998; 41(5): 379-92.
Kickhoefer V A, Rajavel K S, Scheffer G L, Dalton W S, Scheper R J, Rome L H   Vaults are up-regulated in multidrug-resistant cancer cell lines The Journal of biological chemistry, 1998; 273(15): 8971-4.
Raval-Fernandes S, Sawant L A, Aebersold R H, Ducret A, Rome L H   Axonal proteins involved in myelination: characterization of a collagen-like protein Developmental neuroscience, 1997; 19(5): 421-9.
Kickhoefer V A, Vasu S K, Rome L H   Vaults are the answer, what is the question? Trends in cell biology, 1996; 6(5): 174-8.
Malek-Hedayat S, Rome L H   Cloning and sequence of the cDNA encoding the rat oligodendrocyte integrin beta 1 subunit Gene, 1995; 158(2): 287-90.
Vasu S K, Rome L H   Dictyostelium vaults: disruption of the major proteins reveals growth and morphological defects and uncovers a new associated protein The Journal of biological chemistry, 1995; 270(28): 16588-94.
Rome L H   Multidrug resistance: locked in the vault? Nature medicine, 1995; 1(6): 527.
Notterpek L M, Rome L H   A protein involved in central nervous system myelination: localization in the extracellular matrix and induction in neuroblastoma cells Developmental neuroscience, 1994; 16(5-6): 267-78.
Malek-Hedayat S, Rome L H   Expression of a beta 1-related integrin by oligodendroglia in primary culture: evidence for a functional role in myelination The Journal of cell biology, 1994; 124(6): 1039-46.
Notterpek L M, Rome L H   Functional evidence for the role of axolemma in CNS myelination Neuron, 1994; 13(2): 473-85.
Hamilton S P, Rome L H   Stimulation of in vitro myelin synthesis by microglia Glia, 1994; 11(4): 326-35.
Vilalta A, Kickhoefer V A, Rome L H, Johnson D L   The rat vault RNA gene contains a unique RNA polymerase III promoter composed of both external and internal elements that function synergistically The Journal of biological chemistry, 1994; 269(47): 29752-9.
Kickhoefer V A, Rome L H   The sequence of a cDNA encoding the major vault protein from Rattus norvegicus Gene, 1994; 151(1-2): 257-60.
Chugani D C, Rome L H, Kedersha N L   Evidence that vault ribonucleoprotein particles localize to the nuclear pore complex Journal of cell science, 1993; 106 ( Pt 1)(6): 23-9.
Notterpek L M, Bullock P N, Malek-Hedayat S, Fisher R, Rome L H   Myelination in cerebellar slice cultures: development of a system amenable to biochemical analysis Journal of neuroscience research, 1993; 36(6): 621-34.
Grody W W, Kronquist K E, Lee E U, Edmond J, Rome L H   PCR-based cystic fibrosis (CF) carrier screening in a first-year medical student biochemistry laboratory American journal of human genetics, 1993; 53(6): 1352-5.
Kickhoefer V A, Searles R P, Kedersha N L, Garber M E, Johnson D L, Rome L H   Vault ribonucleoprotein particles from rat and bullfrog contain a related small RNA that is transcribed by RNA polymerase III The Journal of biological chemistry, 1993; 268(11): 7868-73.
Vasu S K, Kedersha N L, Rome L H   cDNA cloning and disruption of the major vault protein alpha gene (mvpA) in Dictyostelium discoideum The Journal of biological chemistry, 1993; 268(21): 15356-60.
Malek-Hedayat S, Rome L H   Expression of multiple integrins and extracellular matrix components by C6 glioma cells Journal of neuroscience research, 1992; 31(3): 470-8.
Chugani D C, Kedersha N L, Rome L H   Vault immunofluorescence in the brain: new insights regarding the origin of microglia The Journal of neuroscience : the official journal of the Society for Neuroscience, 1991; 11(1): 256-68.
Kedersha N L, Heuser J E, Chugani D C, Rome L H   Vaults. III. Vault ribonucleoprotein particles open into flower-like structures with octagonal symmetry The Journal of cell biology, 1991; 112(2): 225-35.
Bullock P N, Rome L H   Glass micro-fibers: a model system for study of early events in myelination Journal of neuroscience research, 1990; 27(3): 383-93.
Rome L H, Cardwell M C, Bullock P N, Hamilton S P   Matrix interactions regulating myelinogenesis in cultured oligodendrocytes Advances in experimental medicine and biology, 1990; 265(4): 157-67.
Kedersha N L, Miquel M C, Bittner D, Rome L H   Vaults. II. Ribonucleoprotein structures are highly conserved among higher and lower eukaryotes The Journal of cell biology, 1990; 110(4): 895-901.
Kedersha N L, Rome L H   Vaults: large cytoplasmic RNP's that associate with cytoskeletal elements Molecular biology reports, 1990; 14(2-3): 121-2.
Westcott K R, Rome L H   Cation-independent mannose 6-phosphate receptor contains covalently bound fatty acid Journal of cellular biochemistry, 1988; 38(1): 23-33.
Cardwell M C, Rome L H   Evidence that an RGD-dependent receptor mediates the binding of oligodendrocytes to a novel ligand in a glial-derived matrix The Journal of cell biology, 1988; 107(4): 1541-9.
Cardwell M C, Rome L H   RGD-containing peptides inhibit the synthesis of myelin-like membrane by cultured oligodendrocytes The Journal of cell biology, 1988; 107(4): 1551-9.
Bame K J, Rome L H   Acetyl-CoA: alpha-glucosaminide N-acetyltransferase from rat liver Methods in enzymology, 1987; 138(13): 607-11.
Westcott K R, Searles R P, Rome L H   Evidence for ligand- and pH-dependent conformational changes in liposome-associated mannose 6-phosphate receptor The Journal of biological chemistry, 1987; 262(13): 6101-7.
Lemansky P, Hasilik A, von Figura K, Helmy S, Fishman J, Fine R E, Kedersha N L, Rome L H   Lysosomal enzyme precursors in coated vesicles derived from the exocytic and endocytic pathways The Journal of cell biology, 1987; 104(6): 1743-8.
Bame K J, Rome L H   Acetyl-coenzyme A:alpha-glucosaminide N-acetyltransferase. Evidence for an active site histidine residue The Journal of biological chemistry, 1986; 261(22): 10127-32.
Bame K J, Rome L H   Genetic evidence for transmembrane acetylation by lysosomes Science (New York, N.Y.), 1986; 233(4768): 1087-9.
Tietze F, Rome L H, Butler J D, Harper G S, Gahl W A   Impaired clearance of free cystine from lysosome-enriched granular fractions of I-cell-disease fibroblasts The Biochemical journal, 1986; 237(1): 9-15.
Kedersha N L, Rome L H   Isolation and characterization of a novel ribonucleoprotein particle: large structures contain a single species of small RNA The Journal of cell biology, 1986; 103(3): 699-709.
Rome L H, Hill D F   Lysosomal degradation of glycoproteins and glycosaminoglycans. Efflux and recycling of sulphate and N-acetylhexosamines The Biochemical journal, 1986; 235(3): 707-13.
Kedersha N L, Rome L H   Preparative agarose gel electrophoresis for the purification of small organelles and particles Analytical biochemistry, 1986; 156(1): 161-70.
Kedersha N L, Hill D F, Kronquist K E, Rome L H   Subpopulations of liver coated vesicles resolved by preparative agarose gel electrophoresis The Journal of cell biology, 1986; 103(1): 287-97.
Rome L H, Bullock P N, Chiappelli F, Cardwell M, Adinolfi A M, Swanson D   Synthesis of a myelin-like membrane by oligodendrocytes in culture Journal of neuroscience research, 1986; 15(1): 49-65.
Bame K J, Rome L H   Acetyl coenzyme A: alpha-glucosaminide N-acetyltransferase. Evidence for a transmembrane acetylation mechanism The Journal of biological chemistry, 1985; 260(20): 11293-9.
Hill D F, Bullock P N, Chiappelli F, Rome L H   Binding and internalization of lysosomal enzymes by primary cultures of rat glia Journal of neuroscience research, 1985; 14(1): 35-47.
Mehrabian M, Bame K J, Rome L H   Interaction of rat liver lysosomal membranes with actin The Journal of cell biology, 1984; 99(2): 680-5.
Berg R A, Schwartz M L, Rome L H, Crystal R G   Lysosomal function in the degradation of defective collagen in cultured lung fibroblasts Biochemistry, 1984; 23(10): 2134-8.
Campbell C H, Fine R E, Squicciarini J, Rome L H   Coated vesicles from rat liver and calf brain contain cryptic mannose 6-phosphate receptors The Journal of biological chemistry, 1983; 258(4): 2628-33.
Campbell C H, Rome L H   Coated vesicles from rat liver and calf brain contain lysosomal enzymes bound to mannose 6-phosphate receptors The Journal of biological chemistry, 1983; 258(21): 13347-52.
Campbell C H, Miller A L, Rome L H   Incorporation of mannose 6-phosphate receptors into liposomes. Receptor topography and binding of alpha-mannosidase The Biochemical journal, 1983; 214(2): 413-9.
Rome L H, Hill D F, Bame K J, Crain L R   Utilization of exogenously added acetyl coenzyme A by intact isolated lysosomes The Journal of biological chemistry, 1983; 258(5): 3006-11.
Steiner A W, Rome L H   Assay and purification of a solubilized membrane receptor that binds the lysosomal enzyme alpha-L-iduronidase Archives of biochemistry and biophysics, 1982; 214(2): 681-7.
Rome L H   Uptake and binding of alpha-L-iduronidase Methods in enzymology, 1982; 83(2): 582-7.
Rome L H   alpha-L-iduronidase from human kidney Methods in enzymology, 1982; 83(2): 578-82.
Rome L H, Crain L R   Degradation of mucopolysaccharide in intact isolated lysosomes The Journal of biological chemistry, 1981; 256(21): 10763-8.
Rome L H, Miller J   Butanedione treatment reduces receptor binding of a lysosomal enzyme to cells and membranes Biochemical and biophysical research communications, 1980; 92(3): 986-93.
Rome L H, Weissmann B, Neufeld E F   Direct demonstration of binding of a lysosomal enzyme, alpha-L-iduronidase, to receptors on cultured fibroblasts Proceedings of the National Academy of Sciences of the United States of America, 1979; 76(5): 2331-4.
Rome L H, Garvin A J, Allietta M M, Neufeld E F   Two species of lysosomal organelles in cultured human fibroblasts Cell, 1979; 17(1): 143-53.
Rome L H, Garvin A J, Neufeld E F   Human kidney alpha-L-iduronidase: purification and characterization Archives of biochemistry and biophysics, 1978; 189(2): 344-53.
Neufeld E F, Sando G N, Garvin A J, Rome L H   The transport of lysosomal enzymes Journal of supramolecular structure, 1977; 6(1): 95-101.
Rome L H, Lands W E, Roth G J, Majerus P W   Aspirin as a quantitative acetylating reagent for the fatty acid oxygenase that forms prostaglandins Prostaglandins, 1976; 11(1): 23-30.
Lands W E, Cook H W, Rome L H   Prostaglandin biosynthesis: consequences of oxygenase mechanism upon in vitro assays of drug effectiveness Advances in prostaglandin and thromboxane research, 1976; 1(1): 7-17.
Gao Yifan, Shah Kshitjia, Kwok Ivy, Wang Meng, Rome Leonard H, Mahendra Shaily   Immobilized fungal enzymes: Innovations and potential applications in biodegradation and biosynthesis Biotechnology advances, 2022; 57: 107936.
Gao Yifan, Wang Meng, Shah Kshitija, Singh Kalra Shashank, Rome Leonard H, Mahendra Shaily   Decolorization and detoxification of synthetic dye compounds by laccase immobilized in vault nanoparticles Bioresource technology, 2022; 351: 127040.
Zhang Qin, Jeppesen Dennis K, Higginbotham James N, Graves-Deal Ramona, Trinh Vincent Q, Ramirez Marisol A, Sohn Yoojin, Neininger Abigail C, Taneja Nilay, McKinley Eliot T, Niitsu Hiroaki, Cao Zheng, Evans Rachel, Glass Sarah E, Ray Kevin C, Fissell William H, Hill Salisha, Rose Kristie Lindsey, Huh Won Jae, Washington Mary Kay, Ayers Gregory Daniel, Burnette Dylan T, Sharma Shivani, Rome Leonard H, Franklin Jeffrey L, Lee Youngmin A, Liu Qi, Coffey Robert J   Supermeres are functional extracellular nanoparticles replete with disease biomarkers and therapeutic targets Nature cell biology, 2021; 23(12): 1240-1254.
Rayo Josep, Gregor Rachel, Jacob Nicholas T, Dandela Rambabu, Dubinsky Luba, Yashkin Alex, Aranovich Alexander, Thangaraj Manikandan, Ernst Orna, Barash Eran, Malitsky Sergey, Florea Bogdan I, Krom Bastiaan P, Wiemer Erik A C, Kickhoefer Valerie A, Rome Leonard H, Mathison John C, Kaufmann Gunnar F, Overkleeft Herman S, Cravatt Benjamin F, Zor Tsaffrir, Janda Kim D, Ulevitch Richard J, Kravchenko Vladimir V, Meijler Michael M   Immunoediting role for major vault protein in apoptotic signaling induced by bacterial Proceedings of the National Academy of Sciences of the United States of America, 2021; 118(12): 1240-1254.
Nagasawa Daniel T, Yang Jian, Romiyo Prasanth, Lagman Carlito, Chung Lawrance K, Voth Brittany L, Duong Courtney, Kickhoefer Valerie A, Rome Leonard H, Yang Isaac   Bioengineered recombinant vault nanoparticles coupled with NY-ESO-1 glioma-associated antigens induce maturation of native dendritic cells Journal of neuro-oncology, 2020; 148(1): 1-7.
Voth Brittany L, Pelargos Panayiotis E, Barnette Natalie E, Bhatt Nikhilesh S, Chen Cheng Hao Jacky, Lagman Carlito, Chung Lawrance K, Nguyen Thien, Sheppard John P, Romiyo Prasanth, Mareninov Sergey, Kickhoefer Valerie A, Yong William H, Rome Leonard H, Yang Isaac   Intratumor injection of CCL21-coupled vault nanoparticles is associated with reduction in tumor volume in an in vivo model of glioma Journal of neuro-oncology, 2020; 147(3): 599-605.
Lothe Anjali G, Kalra Shashank Singh, Wang Meng, Mack Elizabeth Erin, Walecka-Hutchison Claudia, Kickhoefer Valerie A, Rome Leonard H, Mahendra Shaily   Vault packaged enzyme mediated degradation of amino-aromatic energetic compounds Chemosphere, 2020; 242(3): 125117.
Fulcher Jennifer A, Tamshen Kyle, Wollenberg Alexander L, Kickhoefer Valerie A, Mrazek Jan, Elliott Julie, Ibarrondo F Javier, Anton Peter A, Rome Leonard H, Maynard Heather D, Deming Timothy, Yang Otto O   Human Vault Nanoparticle Targeted Delivery of Antiretroviral Drugs to Inhibit Human Immunodeficiency Virus Type 1 Infection Bioconjugate chemistry, 2019; 30(8): 2216-2227.
Jeppesen Dennis K, Fenix Aidan M, Franklin Jeffrey L, Higginbotham James N, Zhang Qin, Zimmerman Lisa J, Liebler Daniel C, Ping Jie, Liu Qi, Evans Rachel, Fissell William H, Patton James G, Rome Leonard H, Burnette Dylan T, Coffey Robert J   Reassessment of Exosome Composition Cell, 2019; 177(2): 428-445.e18.
Wang Meng, Chen Yichang, Kickhoefer Valerie A, Rome Leonard H, Allard Patrick, Mahendra Shaily   A Vault-Encapsulated Enzyme Approach for Efficient Degradation and Detoxification of Bisphenol A and Its Analogues ACS sustainable chemistry & engineering, 2019; 7(6): 5808-5817.
Wang Meng, Kickhoefer Valerie A, Rome Leonard H, Foellmer Oliver K, Mahendra Shaily   Synthesis and assembly of human vault particles in yeast Biotechnology and bioengineering, 2018; 115(12): 2941-2950.
Wang Meng, Abad Danny, Kickhoefer Valerie A, Rome Leonard H, Mahendra Shaily   Encapsulation of Exogenous Proteins in Vault Nanoparticles Methods in molecular biology (Clifton, N.J.), 2018; 1798(12): 25-37.

Does this profile need updating? Contact Us