Anna Wu, Ph.D.

Edit your Faculty Profile
Work Titles
CTSI Member, CTSI
California NanoSystems Institute Member, California NanoSystems Institute
UCLA Director, JCCC Cancer Molecular Imaging Professor, Molecular & Medical Pharmacology Member, Molecular Pharmacology GPB Home Area Faculty, Cellular and Molecular Pathology PhD Program Faculty, Crump Institute for Molecular Imaging
Education:
Degrees:
Ph.D., Yale University, New Haven, CT
A.B., Harvard Univeristy, 1975 -

Contact Information:

Work Email Address:

awu@mednet.ucla.edu


Website:

http://faculty.pharmacology.ucla.edu/institution/personnel?personnel_id=45665

Work Phone Number:

(310) 794-5088

Laboratory Address:

Laboratory
Crump Institute
Los Angeles, CA 90095


Office Address:

Office
CNSI 4335, Crump Institute,570 Westwood Plaza
Los Angeles, CA 90095


Publications:

A selected list of publications:

Tavaré Richard, Escuin-Ordinas Helena, Mok Stephen, McCracken Melissa N, Zettlitz Kirstin A, Salazar Felix B, Witte Owen N, Ribas Antoni, Wu Anna M   An Effective Immuno-PET Imaging Method to Monitor CD8-Dependent Responses to Immunotherapy Cancer research, 2016; 76(1): 73-82.
Li Keyu, Zettlitz Kirstin A, Lipianskaya Julia, Zhou Yu, Marks James D, Mallick Parag, Reiter Robert E, Wu Anna M   A fully human scFv phage display library for rapid antibody fragment reformatting Protein engineering, design & selection: PEDS, 2015; 28(10): 307-16.
Örbom Anders, Ahlstedt Jonas, Serén Tom, Auterinen Iiro, Kotiluoto Petri, Hauge HÃ¥vard, Östlund Karl, Olafsen Tove, Wu Anna M, Dahlbom Magnus, Strand Sven-Erik   Characterization of a double-sided silicon strip detector autoradiography system Medical physics, 2015; 42(2): 575-84.
White Jason B, Boucher David L, Zettlitz Kirstin A, Wu Anna M, Sutcliffe Julie L   Development and characterization of an αvβ6-specific diabody and a disulfide-stabilized αvβ6-specific cys-diabody Nuclear medicine and biology, 2015; 42(12): 945-57.
Sonn Geoffrey A, Behesnilian Andrew S, Jiang Ziyue Karen, Zettlitz Kirstin A, Lepin Eric J, Bentolila Laurent, Knowles Scott M, Lawrence Daniel J P, Wu Anna M, Reiter Robert E   Fluorescent Image-Guided Surgery with an Anti-Prostate Stem Cell Antigen (PSCA) Diabody Enables Targeted Resection of Mouse Prostate Cancer Xenografts in Real-Time Clinical cancer research : an official journal of the American Association for Cancer Research, 2015; 5(12): .
Tavaré Richard, McCracken Melissa N, Zettlitz Kirstin A, Salazar Felix B, Olafsen Tove, Witte Owen N, Wu Anna M   Immuno-PET of Murine T Cell Reconstitution Postadoptive Stem Cell Transplantation Using Anti-CD4 and Anti-CD8 Cys-Diabodies Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2015; 56(8): 1258-64.
Freise Amanda C, Wu Anna M   In vivo imaging with antibodies and engineered fragments Molecular immunology, 2015; 67(2 Pt A): 142-52.
Watanabe Rira, Hanaoka Hirofumi, Sato Kazuhide, Nagaya Tadanobu, Harada Toshiko, Mitsunaga Makoto, Kim Insook, Paik Chang H, Wu Anna M, Choyke Peter L, Kobayashi Hisataka   Photoimmunotherapy targeting prostate-specific membrane antigen: are antibody fragments as effective as antibodies? Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2015; 56(1): 140-4.
Rochefort Matthew M, Girgis Mark D, Knowles Scott M, Ankeny Jacob S, Salazar Felix, Wu Anna M, Tomlinson James S   A Mutated Anti-CA19-9 scFv-Fc for Positron Emission Tomography of Human Pancreatic Cancer Xenografts Molecular imaging and biology: MIB: the official publication of the Academy of Molecular Imaging, 2014; 16(5): 721-9.
Li Keyu, Tavaré Richard, Zettlitz Kirstin A, Mumenthaler Shannon M, Mallick Parag, Zhou Yu, Marks James D, Wu Anna M   Anti-MET immunoPET for non-small cell lung cancer using novel fully human antibody fragments Molecular cancer therapeutics, 2014; 13(11): 2607-17.
Knowles Scott M, Tavaré Richard, Zettlitz Kirstin A, Rochefort Matthew M, Salazar Felix B, Jiang Ziyue Karen, Reiter Robert E, Wu Anna M   Applications of immunoPET: using 124I-anti-PSCA A11 minibody for imaging disease progression and response to therapy in mouse xenograft models of prostate cancer Clinical cancer research : an official journal of the American Association for Cancer Research, 2014; 20(24): 6367-78.
Wu Anna M   Engineered antibodies for molecular imaging of cancer Methods (San Diego, Calif.), 2014; 65(1): 139-47.
Tavaré Richard, McCracken Melissa N, Zettlitz Kirstin A, Knowles Scott M, Salazar Felix B, Olafsen Tove, Witte Owen N, Wu Anna M   Engineered antibody fragments for immuno-PET imaging of endogenous CD8+ T cells in vivo Proceedings of the National Academy of Sciences of the United States of America, 2014; 111(3): 1108-13.
Tavaré Richard, Wu Wei H, Zettlitz Kirstin A, Salazar Felix B, McCabe Katelyn E, Marks James D, Wu Anna M   Enhanced immunoPET of ALCAM-positive colorectal carcinoma using site-specific ⁶⁴Cu-DOTA conjugation Protein engineering, design & selection : PEDS, 2014; 27(10): 317-24.
Wilks Moses Q, Knowles Scott M, Wu Anna M, Huang Sung-Cheng   Improved Modeling of In Vivo Kinetics of Slowly Diffusing Radiotracers for Tumor Imaging Journal of nuclear medicine: official publication, Society of Nuclear Medicine, 2014; 55(9): 1539-44.
Watanabe Rira, Sato Kazuhide, Hanaoka Hirofumi, Harada Toshiko, Nakajima Takahito, Kim Insook, Paik Chang H, Wu Anna M, Choyke Peter L, Kobayashi Hisataka   Minibody-indocyanine green based activatable optical imaging probes: the role of short polyethylene glycol linkers ACS medicinal chemistry letters, 2014; 5(4): 411-5.
Viola-Villegas Nerissa Therese, Sevak Kuntal K, Carlin Sean D, Doran Michael G, Evans Henry W, Bartlett Derek W, Wu Anna M, Lewis Jason S   Noninvasive Imaging of PSMA in Prostate Tumors with (89)Zr-Labeled huJ591 Engineered Antibody Fragments: The Faster Alternatives Molecular pharmaceutics, 2014; 11(11): 3965-73.
Williams Lawrence E, Wu Anna M, Kenanova Vania E, Olafsen Tove, Yazaki Paul J   Numerical comparison of iodine-based and indium-based antibody biodistributions Cancer biotherapy & radiopharmaceuticals, 2014; 29(3): 91-8.
Knowles Scott M, Zettlitz Kirstin A, Tavaré Richard, Rochefort Matthew M, Salazar Felix B, Stout David B, Yazaki Paul J, Reiter Robert E, Wu Anna M   Quantitative immunoPET of prostate cancer xenografts with 89Zr- and 124I-labeled anti-PSCA A11 minibody Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2014; 55(3): 452-9.
Sano Kohei, Nakajima Takahito, Ali Towhid, Bartlett Derek W, Wu Anna M, Kim Insook, Paik Chang H, Choyke Peter L, Kobayashi Hisataka   Activatable fluorescent cys-diabody conjugated with indocyanine green derivative: consideration of fluorescent catabolite kinetics on molecular imaging Journal of biomedical optics, 2013; 18(10): 101304.
Girgis Mark D, Federman Noah, Rochefort Matthew M, McCabe Katelyn E, Wu Anna M, Nagy Jon O, Denny Christopher, Tomlinson James S   An engineered anti-CA19-9 cys-diabody for positron emission tomography imaging of pancreatic cancer and targeting of polymerized liposomal nanoparticles The Journal of surgical research, 2013; 185(1): 45-55.
Choe Uh-Joo, Rodriguez April R, Lee Brian S, Knowles Scott M, Wu Anna M, Deming Timothy J, Kamei Daniel T   Endocytosis and intracellular trafficking properties of transferrin-conjugated block copolypeptide vesicles Biomacromolecules, 2013; 14(5): 1458-64.
Widney Daniel P, Olafsen Tove, Wu Anna M, Kitchen Christina M R, Said Jonathan W, Smith Jeffrey B, Peña Guadalupe, Magpantay Larry I, Penichet Manuel L, Martinez-Maza Otoniel   Levels of murine, but not human, CXCL13 are greatly elevated in NOD-SCID mice bearing the AIDS-associated Burkitt lymphoma cell line, 2F7 PloS one, 2013; 8(8): e72414.
Fu Maoyong, Brewer Sarah, Olafsen Tove, Wu Anna M, Gordon Lynn K, Said Jonathan, Braun Jonathan, Wadehra Madhuri   Positron Emission Tomography Imaging of Endometrial Cancer Using Engineered Anti-EMP2 Antibody Fragments Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 2013; 15(1): 68-78.
Knowles Scott M, Wu Anna M   Advances in immuno-positron emission tomography: antibodies for molecular imaging in oncology Journal of clinical oncology : official journal of the American Society of Clinical Oncology, 2012; 30(31): 3884-92.
McCabe Katelyn E, Liu Bin, Marks James D, Tomlinson James S, Wu Hong, Wu Anna M   An Engineered Cysteine-Modified Diabody for Imaging Activated Leukocyte Cell Adhesion Molecule (ALCAM)-Positive Tumors Molecular imaging and biology: MIB: the official publication of the Academy of Molecular Imaging, 2012; 14(3): 336-47.
Andersen Jan Terje, Foss Stian, Kenanova Vania E, Olafsen Tove, Leikfoss Ingvild S, Roopenian Derry C, Wu Anna M, Sandlie Inger   Anti-carcinoembryonic antigen single-chain variable fragment antibody variants bind mouse and human neonatal Fc receptor with different affinities that reveal distinct cross-species differences in serum half-life The Journal of biological chemistry, 2012; 287(27): 22927-37.
Federman Noah, Chan Jason, Nagy Jon O, Landaw Elliot M, McCabe Katelyn, Wu Anna M, Triche Timothy, Kang Hyunggyoo, Liu Bin, Marks James D, Denny Christopher T   Enhanced growth inhibition of osteosarcoma by cytotoxic polymerized liposomal nanoparticles targeting the alcam cell surface receptor Sarcoma, 2012; 2012(2): 126906.
Olafsen Tove, Sirk Shannon J, Olma Sebastian, Shen Clifton K-F, Wu Anna M   ImmunoPET using engineered antibody fragments: fluorine-18 labeled diabodies for same-day imaging Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 2012; 33(3): 669-77.
Girgis Mark D, Kenanova Vania, Olafsen Tove, McCabe Katelyn E, Wu Anna M, Tomlinson James S   Anti-CA19-9 diabody as a PET imaging probe for pancreas cancer The Journal of surgical research, 2011; 170(2): 169-78.
Girgis Mark D, Olafsen Tove, Kenanova Vania, McCabe Katelyn E, Wu Anna M, Tomlinson James S   CA19-9 as a Potential Target for Radiolabeled Antibody-Based Positron Emission Tomography of Pancreas Cancer International journal of molecular imaging, 2011; 2011(1): 834515.
Barat Bhaswati, Kenanova Vania E, Olafsen Tove, Wu Anna M   Evaluation of two internalizing carcinoembryonic antigen reporter genes for molecular imaging Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging, 2011; 13(3): 526-35.
Liu Kan, Lepin Eric J, Wang Ming-Wei, Guo Feng, Lin Wei-Yu, Chen Yi-Chun, Sirk Shannon J, Olma Sebastian, Phelps Michael E, Zhao Xing-Zhong, Tseng Hsian-Rong, Michael van Dam R, Wu Anna M, Shen Clifton K-F   Microfluidic-based 18F-labeling of biomolecules for immuno-positron emission tomography Molecular imaging, 2011; 10(3): 168-76, 1-7.
Liu Kan, Wang Ming-Wei, Lin Wei-Yu, Phung Duy Linh, Girgis Mark D, Wu Anna M, Tomlinson James S, Shen Clifton K-F   Molecular Imaging Probe Development using Microfluidics Current organic synthesis, 2011; 8(4): 473-487.
Olafsen Tove, Elgqvist Jorgen, Wu Anna M   Protein targeting constructs in alpha therapy Current radiopharmaceuticals, 2011; 4(3): 197-213.
Girgis Mark D, Olafsen Tove, Kenanova Vania, McCabe Katelyn E, Wu Anna M, Tomlinson James S   Targeting CEA in Pancreas Cancer Xenografts with a Mutated scFv-Fc Antibody Fragment EJNMMI research, 2011; 1(1): 24.
Morizono Kouki, Xie Yiming, Olafsen Tove, Lee Benhur, Dasgupta Asim, Wu Anna M, Chen Irvin S Y   The soluble serum protein Gas6 bridges virion envelope phosphatidylserine to the TAM receptor tyrosine kinase Axl to mediate viral entry Cell host & microbe, 2011; 9(4): 286-98.
Sherman David J, Kenanova Vania E, Lepin Eric J, McCabe Katelyn E, Kamei Ken-Ichiro, Ohashi Minori, Wang Shutao, Tseng Hsian-Rong, Wu Anna M, Behrenbruch Christian P   A differential cell capture assay for evaluating antibody interactions with cell surface targets Analytical biochemistry, 2010; 401(2): 173-81.
Wong Jeffrey Y C, Raubitschek Andrew, Yamauchi Dave, Williams Lawrence E, Wu Anna M, Yazaki Paul, Shively John E, Colcher David, Somlo George   A pretherapy biodistribution and dosimetry study of indium-111-radiolabeled trastuzumab in patients with human epidermal growth factor receptor 2-overexpressing breast cancer Cancer biotherapy & radiopharmaceuticals, 2010; 25(4): 387-94.
Lepin Eric J, Leyton Jeffrey V, Zhou Yu, Olafsen Tove, Salazar Felix B, McCabe Katelyn E, Hahm Scott, Marks James D, Reiter Robert E, Wu Anna M   An affinity matured minibody for PET imaging of prostate stem cell antigen (PSCA)-expressing tumors European journal of nuclear medicine and molecular imaging, 2010; 37(8): 1529-38.
Lepin EJ, Leyton JV, Zhou Y, Olafsen T, Salazar FB, McCabe KE, Hahm S, Marks JD, Reiter RE, Wu AM   An affinity matured minibody for PET imaging of prostate stem cell antigen (PSCA)-expressing tumors Eur J Nucl Med Mol Imaging, 2010; 37(8): 1529-38.
Olafsen Tove, Wu Anna M   Antibody vectors for imaging Seminars in nuclear medicine, 2010; 40(3): 167-81.
Olafsen T, Sirk SJ, Betting DJ, Kenanova VE, Bauer KB, Ladno W, Raubitschek AA, Timmerman JM, Wu AM   ImmunoPET imaging of B-cell lymphoma using 124I-anti-CD20 scFv dimers (diabodies) Protein Eng Des Sel, 2010; 23(4): 243-9.
Olafsen Tove, Sirk Shannon J, Betting David J, Kenanova Vania E, Bauer Karl B, Ladno Waldemar, Raubitschek Andrew A, Timmerman John M, Wu Anna M   ImmunoPET imaging of B-cell lymphoma using 124I-anti-CD20 scFv dimers (diabodies) Protein engineering, design & selection : PEDS, 2010; 23(4): 243-9.
Gagnon Pete, Cheung Chia-Wei, Lepin Eric J, Wu Anna M, Sherman Mark A, Raubitschek Andrew A, Yazaki Paul J   Minibodies and Multimodal Chromatography Methods: A Convergence of Challenge and Opportunity BioProcess international, 2010; 8(2): 26-35.
McCabe Katelyn E, Wu Anna M   Positive progress in immunoPET--not just a coincidence Cancer biotherapy & radiopharmaceuticals, 2010; 25(3): 253-61.
Aweda TA, Beck HE, Wu AM, Wei LH, Weber WA, Meares CF   Rates and equilibria for probe capture by an antibody with infinite affinity Bioconjug Chem, 2010; 21(4): 784-91.
Kenanova Vania E, Olafsen Tove, Salazar Felix B, Williams Lawrence E, Knowles Scott, Wu Anna M   Tuning the serum persistence of human serum albumin domain III:diabody fusion proteins Protein engineering, design & selection : PEDS, 2010; 23(10): 789-98.
Olafsen T, Young SG, Davies BS, Beigneux AP, Kenanova VE, Voss C, Young G, Wong KP, Barnes RH 2nd, Tu Y, Weinstein MM, Nobumori C, Huang SC, Goldberg IJ, Bensadoun A, Wu AM, Fong LG   Unexpected expression pattern for glycosylphosphatidylinositol-anchored HDL-binding protein 1 (GPIHBP1) in mouse tissues revealed by positron emission tomography scanning J Biol Chem, 2010; 285(50): 39239-48.
Wu, A. M.   Antibodies and Antimatter: The Resurgence of Immuno-PET Journal of Nuclear Medicine, 2009; 50(1): 2-5.
Shimazaki, K. Chan, A. M. Moniz, R. J. Wadehra, M. Nagy, A. Coulam, C. P. Mareninov, S. Lepin, E. M. Wu, A. M. Kelly, K. A. Braun, J. Gordon, L. K.   Blockade of epithelial membrane protein 2 (EMP2) abrogates infection of Chlamydia muridarum murine genital infection model Fems Immunology and Medical Microbiology, 2009; 55(2): 240-249.
Afshar Sepideh, Olafsen Tove, Wu Anna M, Morrison Sherie L   Characterization of an engineered human purine nucleoside phosphorylase fused to an anti-her2/neu single chain Fv for use in ADEPT Journal of experimental & clinical cancer research : CR, 2009; 28(12): 147.
Barat Bhaswati, Sirk Shannon J, McCabe Katelyn E, Li Jianqing, Lepin Eric J, Remenyi Roland, Koh Ai Leen, Olafsen Tove, Gambhir Sanjiv S, Weiss Shimon, Wu Anna M   Cys-diabody quantum dot conjugates (immunoQdots) for cancer marker detection Bioconjugate chemistry, 2009; 20(8): 1474-81.
Leyton, J.V., Olafsen, T., Sherman, M.A., Bauer, K.B., Aghajanian, P., Reiter, R.E., and Wu, A.M.   Engineered humanized diabodies for microPET imaging of prostate stem cell antigen (PSCA)-expressing tumors Protein Engineering Design & Selection, 2009; 22: 209-216.
Vaidyanathan Ganesan, Jestin Emmanuelle, Olafsen Tove, Wu Anna M, Zalutsky Michael R   Evaluation of an anti-p185(HER2) (scFv-C(H)2-C(H)3)2 fragment following radioiodination using two different residualizing labels: SGMIB and IB-Mal-D-GEEEK Nuclear medicine and biology, 2009; 36(6): 671-80.
Frank Richard T, Edmiston Marissa, Kendall Stephen E, Najbauer Joseph, Cheung Chia-Wei, Kassa Thewodros, Metz Marianne Z, Kim Seung U, Glackin Carlotta A, Wu Anna M, Yazaki Paul J, Aboody Karen S   Neural stem cells as a novel platform for tumor-specific delivery of therapeutic antibodies PloS one, 2009; 4(12): e8314.
Schipper, M. L. Iyer, G. Koh, A. L. Cheng, Z. Ebenstein, Y. Aharoni, A. Keren, S. Bentolila, L. A. Li, J. Q. Rao, J. H. Chen, X. Y. Banin, U. Wu, A. M. Sinclair, R. Weiss, S. Gambhir, S. S.   Particle Size, Surface Coating, and PEGylation Influence the Biodistribution of Quantum Dots in Living Mice Small, 2009; 5(1): 126-134.
Olafsen Tove, Betting David, Kenanova Vania E, Salazar Felix B, Clarke Pat, Said Jonathan, Raubitschek Andrew A, Timmerman John M, Wu Anna M   Recombinant anti-CD20 antibody fragments for small-animal PET imaging of B-cell lymphomas Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2009; 50(9): 1500-8.
Kenanova, V.E., Barat, B., Olafsen, T., Chatziioannou, A., Herschman, H.R., Braun, J., and Wu, A.M.   Recombinant carcinoembryonic antigen as a reporter gene for molecular imaging Eur. J. Nucl. Med. Molec. Imaging, 2009; 36(1): 104-114.
Brown Robert H, Irvin Charles G, Allen Gilman B, Shapiro Steven D, Martin William J, Kolb Martin R J, Hyde Dallas M, Nieman Gary F, Cody Dianna D, Ishii Masaru, Kadlecek Stephen J, Driehuys Bastiaan, Rizi Rahim R, Wu Anna M, Weber Wolfgang A, Stout David B, Stout David B   An official ATS conference proceedings: advances in small-animal imaging application to lung pathophysiology Proceedings of the American Thoracic Society, 2008; 5(5): 591-600.
Wu, A. M. Olafsen, T.   Antibodies for molecular imaging of cancer Cancer Journal, 2008; 14(3): 191-197.
Shimazaki, K., Lepin, E.J., Wei, B., Nagy, A., Coulum, P., Mareninov, S., Fu, M., Wu, A.M., Marks, J.D., Braun, J., Gordon, L.K., and Wadehra, M.   Diabodies targeting epithelial membrane protein 2 reduce tumorigenicity of human endometrial cancer cell lines Clin. Cancer Res, 2008; 14: 7367-7377.
Wei, L. H. Olafsen, T. Radu, C. Hildebrandt, I. J. Mccoy, M. R. Phelps, M. E. Meares, C. Wu, A. M. Czernin, J. Weber, W. A.   Engineered Antibody Fragments with Infinite Affinity as Reporter Genes for PET Imaging Journal of Nuclear Medicine, 2008; 49(11): 1828-1835.
Leyton, J. V. Olafsen, T. Lepin, E. J. Hahm, S. Bauer, K. B. Reiter, R. E. Wu, A. M.   Humanized Radioiodinated Minibody For Imaging of Prostate Stem Cell Antigen-Expressing Tumors Clinical Cancer Research, 2008; 14(22): 7488-7496.
Sirk, S., Olafsen, T., Barat, B., Bauer, K. B., McCabe, K.E., and Wu, A.M.   Site-specific thiol-mediated conjugation of fluorescent probes to cysteine-modified diabodies targeting CD20 or HER2 Bioconjugate Chemistry, 2008; 19: 2527-2534.
Venisnik, K. M. Olafsen, T. Gambhir, S. S. Wu, A. M.   Fusion of Gaussia luciferase to an engineered anti-carcinoembryonic antigen (CEA) antibody for in vivo optical imaging Molecular Imaging and Biology, 2007; 9(5): 267-277.
Barat, B. Wu, A. M.   Metabolic biotinylation of recombinant antibody by biotin ligase retained in the endoplasmic reticulum Biomolecular Engineering, 2007; 24(3): 283-291.
Schipper, M.L., Cheng, Z., Lee, S.-W., Bentolila, L.A., Iyer, G., Rao, J., Chen, X., Wu, A.M., Weiss, S., and Gambhir, S.S.   MicroPET-based biodistribution of quantum dots in living mice J. Nucl. Med, 2007; 48: 1511-1518.
Cai, W Olafsen, T Zhang, X Cao, Q Gambhir, SS Williams, LE Wu, AM Chen, X   PET imaging of colorectal cancer in xenograft-bearing mice by use of an 18F-labeled T84.66 anti-carcinoembryonic antigen diabody Journal of nuclear medicine : official publication, Society of Nuclear Medicine, 2007; 48(2): 304-10.
Kenanova, V. Olafsen, T. Williams, L. E. Ruel, N. H. Longmate, J. Yazaki, P. J. Shively, J. E. Colcher, D. Raubitschek, A. A. Wu, A. M.   Radioiodinated versus radiometal-labeled anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments: Optimal pharmacokinetics for therapy Cancer Research, 2007; 67(2): 718-726.
Loening, AM Wu, AM Gambhir, SS   Red-shifted Renilla reniformis luciferase variants for imaging in living subjects Nature Methods, 2007; 4(8): 641-3.
Olafsen, T. Gu, Z. Sherman, M. A. Leyton, J. V. Witkosky, M. E. Shively, J. E. Raubitschek, A. A. Morrison, S. L. Wu, A. M. Reiter, R. E.   Targeting, imaging, and therapy using a humanized antiprostate stem cell antigen (PSCA) antibody Journal of Immunotherapy, 2007; 30(4): 396-405.
Wong, J.Y.C., Chu, D.Z., Williams, L.E., Liu, A., Zhan, J., Yamauchi, D.M., Wilczynski, S., Wu, A.M., Yazaki, P.J., Shively, J.E., Leong, L., Raubitschek, A.A.   A Phase I trial of 90Y-DOTA-anti-CEA chimeric T84.66 (cT84.66) radioimmunotherapy in patients with metastatic CEA-producing malignancies Cancer Biother. Radiopharm, 2006; 21: 88-100.
Ferl, G. Z. Kenanova, V. Wu, A. M. DiStefano, J. J.   A two-tiered physiologically based model for dually labeled single-chain Fv-Fc antibody fragments Molecular Cancer Therapeutics, 2006; 5(6): 1550-1558.
Venisnik, K. M. Olafsen, T. Loening, A. M. Iyer, M. Gambhir, S. S. Wu, A. M.   Bifunctional antibody-Renilla luciferase fusion protein for in vivo optical detection of tumors Protein Engineering Design & Selection, 2006; 19(10): 453-460.
Loening, A. M. Fenn, T. D. Wu, A. M. Gambhir, S. S.   Consensus guided mutagenesis of Renilla luciferase yields enhanced stability and light output Protein Engineering Design & Selection, 2006; 19(9): 391-400.
Li, L. Yazaki, P. J. Anderson, A. L. Crow, D. Colcher, D. Wu, A. M. Williams, L. E. Wong, J. Y. C. Raubitschek, A. Shively, J. E.   Improved biodistribution and radioimmunoimaging with poly(ethylene glycol)-DOTA-conjugated anti-CEA diabody Bioconjugate Chemistry, 2006; 17(1): 68-76.
Sui, G. D. Wang, J. Y. Lee, C. C. Lu, W. X. Lee, S. P. Leyton, J. V. Wu, A. M. Tseng, H. R.   Solution-phase surface modification in intact poly(dimethylsiloxane) microfluidic channels Analytical Chemistry, 2006; 78(15): 5543-5551.
Kenanova, V.E. and Wu, A.M.   Tailoring antibodies for radionuclide delivery Exp. Opin. Drug Delivery, 2006; 3: 53-70.
Olafsen, T Kenanova, VE Wu, AM   Tunable pharmacokinetics: modifying the in vivo half-life of antibodies by directed mutagenesis of the Fc fragment Nature protocols, 2006; 1(4): 2048-60.
Ferl, G. Z. Wu, A. M. Distefano, J. J.   A predictive model of therapeutic monoclonal antibody dynamics and regulation by the neonatal fc receptor (FcRn) Annals of Biomedical Engineering, 2005; 33(11): 1640-1652.
Huang, S. C. Truong, D. Wu, H. M. Chatziioannou, A. F. Shao, W. Wu, A. M. Phelps, M. E.   An Internet-based "kinetic imaging system" (KIS) for MicroPET Molecular Imaging and Biology, 2005; 7(5): 330-341.
Wu, A. M. Senter, P. D.   Arming antibodies: prospects and challenges for immunoconjugates Nature Biotechnology, 2005; 23(9): 1137-1146.
Olafsen, T. Kenanova, V. E. Sundaresan, G. Anderson, A. L. Crow, D. Yazaki, P. J. Li, L. Press, M. F. Williams, L. E. Wong, J. Y. C. Raubitschek, A. A. Shively, J. E. Wu, A. M.   Optimizing radiolabeled engineered anti-p185(HER2) antibody fragments for in vivo imaging Cancer Research, 2005; 65(13): 5907-5916.
Michalet, X. Pinaud, F. F. Bentolila, L. A. Tsay, J. M. Doose, S. Li, J. J. Sundaresan, G. Wu, A. M. Gambhir, S. S. Weiss, S.   Quantum dots for live cells, in vivo imaging, and diagnostics Science, 2005; 307(5709): 538-544.
Bentolila LA, Michalet X, Pinaud FF, Tsay JM, Doose S, Li JJ, Sundaresan G, Wu AM, Gambhir SS, Weiss S   Quantum dots for molecular imaging and cancer medicine Discov Med, 2005; 5(26): 213-8.
Kenanova, V. Olafsen, T. Crow, D. M. Sundaresan, G. Subbarayan, M. Carter, N. H. Ikle, D. N. Yazaki, P. J. Chatziioannou, A. F. Gambhir, S. S. Williams, L. E. Shively, J. E. Colcher, D. Raubitschek, A. A. Wu, A. M.   Tailoring the pharmacokinetics and positron emission tomography imaging properties of anti-carcinoembryonic antigen single-chain Fv-Fc antibody fragments Cancer Research, 2005; 65(2): 622-631.
Olafsen T, Tan GJ, Cheung CW, Yazaki PJ, Park JM, Shively JE, Williams LE, Raubitschek AA, Press MF, Wu AM   Characterization of engineered anti-p185HER-2 (scFv-CH3)2 antibody fragments (minibodies) for tumor targeting Protein engineering, design & selection : PEDS, 2004; 17(4): 315-23.
Olafsen T, Cheung CW, Yazaki PJ, Li L, Sundaresan G, Gambhir SS, Sherman MA, Williams LE, Shively JE, Raubitschek AA, Wu AM   Covalent disulfide-linked anti-CEA diabody allows site-specific conjugation and radiolabeling for tumor targeting applications Protein engineering, design & selection : PEDS, 2004; 17(1): 21-7.
Wu Anna M   Engineering multivalent antibody fragments for in vivo targeting Methods in molecular biology (Clifton, N.J.), 2004; 248(15): 209-25.
Yazaki Paul J, Wu Anna M   Expression of recombinant antibodies in mammalian cell lines Methods in molecular biology (Clifton, N.J.), 2004; 248(15): 255-68.
Yazaki, P. J. Sherman, M. A. Shively, J. E. Ikle, D. Williams, L. E. Wong, J. Y. C. Colcher, D. Wu, A. M. Raubitschek, A. A.   Humanization of the anti-CEA T84.66 antibody based on crystal structure data Protein Engineering Design & Selection, 2004; 17(5): 481-489.
Wong, J. Y. C. Chu, D. Z. Williams, L. E. Yamauchi, D. M. Ikle, D. N. Kwok, C. S. Liu, A. Wilczynski, S. Colcher, D. Yazaki, P. J. Shively, J. E. Wu, A. M. Raubitschek, A. A.   Pilot trial evaluating an I-123-labeled 80-kilodalton engineered anticarcinoembryonic antigen antibody fragment (cT84.66 minibody) in patients with colorectal cancer Clinical Cancer Research, 2004; 10(15): 5014-5021.
Sundaresan G, Yazaki PJ, Shively JE, Finn RD, Larson SM, Raubitschek AA, Williams LE, Chatziioannou AF, Gambhir SS, Wu AM   124I-labeled engineered anti-CEA minibodies and diabodies allow high-contrast, antigen-specific small-animal PET imaging of xenografts in athymic mice Journal of nuclear medicine: Official Publication, Society of Nuclear Medicine, 2003; 44(12): 1962-9.
Wong Jeffrey Y C, Shibata Stephen, Williams Lawrence E, Kwok Cheuk S, Liu An, Chu David Z, Yamauchi Dave M, Wilczynski Sharon, Ikle David N, Wu Anna M, Yazaki Paul J, Shively John E, Doroshow James H, Raubitschek Andrew A   A Phase I trial of 90Y-anti-carcinoembryonic antigen chimeric T84.66 radioimmunotherapy with 5-fluorouracil in patients with metastatic colorectal cancer Clinical cancer research : an official journal of the American Association for Cancer Research, 2003; 9(16 Pt 1): 5842-52.
Ye JJ, Liang SJ, Guo N, Li SL, Wu AM, Giannini S, Sachdev D, Yee D, Brünner N, Ikle D, Fujita-Yamaguchi Y   Combined effects of tamoxifen and a chimeric humanized single chain antibody against the type I IGF receptor on breast tumor growth in vivo Horm Metab Res, 2003; 35(11-12): 836-42.
Yazaki Paul J, Wu Anna M   Construction and characterization of minibodies for imaging and therapy of colorectal carcinomas Methods in molecular biology (Clifton, N.J.), 2003; 207(16 Pt 1): 351-64.
Jensen MC, Cooper LJ, Wu AM, Forman SJ, Raubitschek A   Engineered CD20-specific primary human cytotoxic T lymphocytes for targeting B-cell malignancy Cytotherapy, 2003; 5(2): 131-8.
Ray, P. Wu, A. M. Gambhir, S. S.   Optical bioluminescence and positron emission tomography imaging of a novel fusion reporter gene in tumor xenografts of living mice Cancer Research, 2003; 63(6): 1160-1165.
Carmichael JA, Power BE, Garrett TP, Yazaki PJ, Shively JE, Raubischek AA, Wu AM, Hudson PJ   The crystal structure of an anti-CEA scFv diabody assembled from T84.66 scFvs in V(L)-to-V(H) orientation: implications for diabody flexibility J Mol Biol, 2003; 326(2): 341-51.
Li L, Olafsen T, Anderson AL, Wu A, Raubitschek AA, Shively JE   Reduction of kidney uptake in radiometal labeled peptide linkers conjugated to recombinant antibody fragments. Site-specific conjugation of DOTA-peptides to a Cys-diabody Bioconjug Chem, 2002; 13(5): 985-95.
Dickersin K, Braun L, Mead M, Millikan R, Wu AM, Pietenpol J, Troyan S, Anderson B, Visco F   Development and implementation of a science training course for breast cancer activists: Project LEAD (leadership, education and advocacy development) Health Expect, 2001; 4(4): 213-20.
Yazaki PJ, Shively L, Clark C, Cheung CW, Le W, Szpikowska B, Shively JE, Raubitschek AA, Wu AM   Mammalian expression and hollow fiber bioreactor production of recombinant anti-CEA diabody and minibody for clinical applications J Immunol Methods, 2001; 253(1-2): 195-208.
Wu AM, Tan GJ, Sherman MA, Clarke P, Olafsen T, Forman SJ, Raubitschek AA   Multimerization of a chimeric anti-CD20 single-chain Fv-Fc fusion protein is mediated through variable domain exchange Protein Eng, 2001; 14(12): 1025-33.
Williams LE, Wu AM, Yazaki PJ, Liu A, Raubitschek AA, Shively JE, Wong JY   Numerical selection of optimal tumor imaging agents with application to engineered antibodies Cancer Biother Radiopharm, 2001; 16(1): 25-35.
Wu, A. M.   Tools for pretargeted radioimmunotherapy Cancer Biotherapy and Radiopharmaceuticals, 2001; 16(2): 103-108.
Yazaki PJ, Wu AM, Tsai SW, Williams LE, Ikler DN, Wong JY, Shively JE, Raubitschek AA   Tumor targeting of radiometal labeled anti-CEA recombinant T84.66 diabody and t84.66 minibody: comparison to radioiodinated fragments Bioconjug Chem, 2001; 12(2): 220-8.
Wong JYC, Chu DZ, Yamauchi DM, Williams LE, Liu A, Wilczynski S, Wu AM, Shively JE, Doroshow JH, Raubitschek AA   A phase I radioimmunotherapy trial evaluating 90yttrium-labeled anti-carcinoembryonic antigen (CEA) chimeric T84.66 in patients with metastatic CEA-producing malignancies Clin Cancer Res, 2000; 6(10): 3855-63.
Tsai SW, Sun Y, Williams LE, Raubitschek AA, Wu AM, Shively JE   Biodistribution and radioimmunotherapy of human breast cancer xenografts with radiometal-labeled DOTA conjugated anti-HER2/neu antibody 4D5 Bioconjug Chem, 2000; 11(3): 327-34.
Wu AM, Yazaki PJ   Designer genes: recombinant antibody fragments for biological imaging Q J Nucl Med, 2000; 44(3): 268-83.
Wu, A. M. Yazaki, P. J. Tsai, S. W. Nguyen, K. Anderson, A. L. McCarthy, D. W. Welch, M. J. Shively, J. E. Williams, L. E. Raubitschek, A. A. Wong, J. Y. C. Toyokuni, T. Phelps, M. E. Gambhir, S. S.   High-resolution microPET imaging of carcino-embryonic antigen-positive xenografts by using a copper-64-labeled engineered antibody fragment Proceedings of the National Academy of Sciences of the United States of America, 2000; 97(15): 8495-8500.
Jensen MC, Clarke P, Tan G, Wright C, Chung-Chang W, Clark TN, Zhang F, Slovak ML, Wu AM, Forman SJ, Raubitschek A   Human T lymphocyte genetic modification with naked DNA Mol Ther, 2000; 1(1): 49-55.
Li SL, Liang SJ, Guo N, Wu AM, Fujita-Yamaguchi Y   Single-chain antibodies against human insulin-like growth factor I receptor: expression, purification, and effect on tumor growth Cancer Immunol Immunother, 2000; 49(4-5): 243-52.
Williams LE, Liu A, Wu AM, Yazaki PJ, Yamauchi DM, Lopatin G, Raubitschek AA, Wong JY   Truncation of blood curves to enhance imaging and therapy with monoclonal antibodies Med Phys, 2000; 27(5): 988-94.
Wong JY, Somlo G, Odom-Maryon T, Williams LE, Liu A, Yamauchi D, Wu AM, Yazaki P, Wilczynski S, Shively JE, Forman S, Doroshow JH, Raubitschek AA   Initial clinical experience evaluating Yttrium-90-chimeric T84.66 anticarcinoembryonic antigen antibody and autologous hematopoietic stem cell support in patients with carcinoembryonic antigen-producing metastatic breast cancer Clin Cancer Res, 1999; 5(10 Suppl): 3224s-3231s.
Jensen M, Tan G, Forman S, Wu AM, Raubitschek A   CD20 is a molecular target for scFvFc:zeta receptor redirected T cells: implications for cellular immunotherapy of CD20+ malignancy Biol Blood Marrow Transplant, 1998; 4(2): 75-83.
Wong JY, Chu DZ, Yamauchi D, Odom-Maryon TL, Williams LE, Liu A, Esteban JM, Wu AM, Primus FJ, Beatty JD, Shively JE, Raubitschek AA   Dose escalation trial of indium-111-labeled anti-carcinoembryonic antigen chimeric monoclonal antibody (chimeric T84.66) in presurgical colorectal cancer patients J Nucl Med, 1998; 39(12): 2097-104.
You YH, Hefta LJ, Yazaki PJ, Wu AM, Shively JE   Expression, purification, and characterization of a two domain carcinoembryonic antigen minigene (N-A3) in pichia pastoris. The essential role of the N-domain Anticancer Res, 1998; 18(5A): 3193-201.
Wong JY, Thomas GE, Yamauchi D, Williams LE, Odom-Maryon TL, Liu A, Esteban JM, Neumaier M, Dresse S, Wu AM, Primus FJ, Shively JE, Raubitschek AA   Clinical evaluation of indium-111-labeled chimeric anti-CEA monoclonal antibody J Nucl Med, 1997; 38(12): 1951-9.
Hu S, Shively L, Raubitschek A, Sherman M, Williams LE, Wong JY, Shively JE, Wu AM   Minibody: A novel engineered anti-carcinoembryonic antigen antibody fragment (single-chain Fv-CH3) which exhibits rapid, high-level targeting of xenografts Cancer Res, 1996; 56(13): 3055-61.
Wu AM, Chen W, Raubitschek A, Williams LE, Neumaier M, Fischer R, Hu SZ, Odom-Maryon T, Wong JY, Shively JE   Tumor localization of anti-CEA single-chain Fvs: improved targeting by non-covalent dimers Immunotechnology, 1996; 2(1): 21-36.
Williams LE, Liu A, Wu AM, Odom-Maryon T, Chai A, Raubitschek AA, Wong JY   Figures of merit (FOMs) for imaging and therapy using monoclonal antibodies Med Phys, 1995; 22(12): 2025-7.
Wong JY, Williams LE, Yamauchi DM, Odom-Maryon T, Esteban JM, Neumaier M, Wu AM, Johnson DK, Primus FJ, Shively JE, et al   Initial experience evaluating 90yttrium-radiolabeled anti-carcinoembryonic antigen chimeric T84.66 in a phase I radioimmunotherapy trial Cancer Res, 1995; 55(23 Suppl): 5929s-5934s.
Ehsani A, Low J, Wallace RB, Wu AM   Characterization of a new allele of the human ERBB2 gene by allele-specific competition hybridization Genomics, 1993; 15(2): 426-9.
Kashani-Sabet M, Funato T, Tone T, Jiao L, Wang W, Yoshida E, Kashfinn BI, Shitara T, Wu AM, Moreno JG, et al.   Reversal of the malignant phenotype by an anti-ras ribozyme Antisense Res Dev, 1992; 2(1): 3-15.
Wu AM, Ben-Ezra J, Winberg C, Colombero AM, Rappaport H   Analysis of antigen receptor gene rearrangements in ethanol and formaldehyde-fixed, paraffin-embedded specimens Lab Invest, 1990; 63(1): 107-14.
Wu AM, Winberg CD, Sheibani K, Colombero AM, Wallace RB, Rappaport H   Genotype and phenotype: a practical approach to the immunogenetic analysis of lymphoproliferative disorders Hum Pathol, 1990; 21(11): 1132-41.
DasGupta C, Wu AM, Kahn R, Cunningham RP, Radding CM   Concerted strand exchange and formation of Holliday structures by E. coli RecA protein Cell, 1981; 25(2): 507-16.
Cunningham RP, Wu AM, Shibata T, DasGupta C, Radding CM   Homologous pairing and topological linkage of DNA molecules by combined action of E. coli RecA protein and topoisomerase I Cell, 1981; 24(1): 213-23.
Wu AM, Christie GE, Platt T   Tandem termination sites in the tryptophan operon of Escherichia coli Proc Natl Acad Sci U S A, 1981; 78(5): 2913-7.
Yanofsky C, Platt T, Crawford IP, Nichols BP, Christie GE, Horowitz H, VanCleemput M, Wu AM   The complete nucleotide sequence of the tryptophan operon of Escherichia coli Nucleic Acids Res, 1981; 9(24): 6647-68.
Wu AM, Chapman AB, Platt T, Guarente LP, Beckwith J   Deletions of distal sequence after termination of transcription at the end of the tryptophan operon in E. coli Cell, 1980; 19(4): 829-36.
Guarente LP, Beckwth J, Wu AM, Platt T   A mutation distal to the messenger RNA endpoint reduces transcription termination in the tryptophan operon in Escherichia coli J Mol Biol, 1979; 133(1): 189-97.
Wu AM, Platt T   Transcription termination: nucleotide sequence at 3' end of tryptophan operon in Escherichia coli Proc Natl Acad Sci U S A, 1978; 75(11): 5442-6.

Does this profile need updating? Contact Us