Harumi Kasamatsu, Ph.D.


Work Titles
UCLA Professor, Molecular, Cell, and Developmental Biology
Education:
Degrees:
Ph.D.

Contact Information:

Work Email Address:

harumi_k@mbi.ucla.edu


Website:



Work Phone Number:

(310) 825-3048

Laboratory Address:

Laboratory
464 Boyer Hall
611 E. Charles E. Young Dr.
UCLA
Los Angeles, CA 90095


Work Address:

Office
456 Boyer Hall
611 E. Charles E. Young Dr.
UCLA
Los Angeles, CA 90095


Detailed Biography:

Morphogenesis of a DNA tumor virus. Recent studies appear to associate natural SV40 strains with human brain tumors of early childhood. The goal of our research is to understand the pathways that contribute to the formation of a DNA tumor virus, namely, SV40, and to understand how tumor antigens play a role in viral morphogenesis. Our work concerns defining the individual protein domains of SV40 structural proteins that determine the individual steps in the morphogenesis and relating these domains to their biological activities. We are using various molecular biological, genetic, biochemical,cell biological and immunological approaches to ask various questions, specifically: (1) What is the structural determinant for the nuclear targeting of an infectious virion particle, and what is the mechanism of the targeting? (2) What components of viral proteins are important for viral DNA targeting? (3) How infecting virions move tiwards the nucleus in the cytoplasm? (4) How do viral structural proteins fold into correct, functional structures? (5) How are individual viral proteins intracellularly targeted to the nucleus following their synthesis? (6) What are the determinants of individual viral proteins that specify protein-protein interactions? (7) Where and how do the interactions of each viral protein leading to virion assembly occur? (8) What are the determinants that initiate genome packaging and how does the packaging occur? A basic understanding of the functional determinants is indispensable toward understanding which ones mediate infection and which ones prevent the spread of the DNA tumor viruses. For example, successful identification of the specific viral components or viral protein domains that mediate effective nuclear targeting of SV40 DNA may lead to the development of a general, effective means to exogenously introduce DNA or tumor killing agent into mammalian cells.

Publications:

Watanabe Marika, Phamduong Ellen, Huang Chu-Han, Itoh Noriko, Bernal Janie, Nakanishi Akira, Rundell Kathleen, Gjoerup Ole, Kasamatsu Harumi   Formation of covalently modified folding intermediates of simian virus 40 Vp1 in large T antigen-expressing cells Journal of virology, 2013; 87(9): 5053-64.
Li Peggy P, Itoh Noriko, Watanabe Marika, Shi Yunfan, Liu Peony, Yang Hui-Jung, Kasamatsu Harumi   Association of simian virus 40 vp1 with 70-kilodalton heat shock proteins and viral tumor antigens Journal of virology, 2009; 83(1): 37-46.
Li Peggy P, Nguyen Albert P, Qu Qiumin, Jafri Qumber H, Aungsumart Saharat, Cheng R Holland, Kasamatsu Harumi   Importance of calcium-binding site 2 in simian virus 40 infection Journal of virology, 2007; 81(11): 6099-105.
Nakanishi Akira, Itoh Noriko, Li Peggy P, Handa Hiroshi, Liddington Robert C, Kasamatsu Harumi   Minor capsid proteins of simian virus 40 are dispensable for nucleocapsid assembly and cell entry but are required for nuclear entry of the viral genome Journal of virology, 2007; 81(8): 3778-85.
Nakanishi Akira, Li Peggy P, Qu Qiumin, Jafri Qumber H, Kasamatsu Harumi   Molecular dissection of nuclear entry-competent SV40 during infection Virus research, 2007; 124(1-2): 226-30.
Nakanishi Akira, Nakamura Akiko, Liddington Robert, Kasamatsu Harumi   Identification of amino acid residues within simian virus 40 capsid proteins Vp1, Vp2, and Vp3 that are required for their interaction and for viral infection Journal of virology, 2006; 80(18): 8891-8.
Kasamatsu, H., Woo, J., Nakamura, A., Muller, P., Tevethia, M.J., and Liddington, R.C.   A structural rationale for SV40 Vp1 temperature-sensitive mutants and their complementation, Protein Science, 2006; 15: 2207-2213.
Kasamatsu, H., Woo, J., Nakamura, A., Muller, P., Tevethia, M.J., and Liddington, R.C.   A structural rationale for SV40 Vp1 temperature-sensitive mutants and their complementation, Protein Science, 2006; 15: 2207-2213.
Nakanishi, A., Nakamura, A., Liddington, R., and Kasamatsu, H.   Identification of amino acid residues within simian virus 40 capsid proteins Vp1, Vp2, and Vp3 that are required for their interaction and for viral infection, Journal of Virology, 2006; 80: 8891-8898.
Nakanishi, A., Li, P.P., Qu, Q., Jafri, Q.H., and Kasamatsu, H.   Molecular dissection of nuclear entry-competent SV40 during infection, Virus Research, 2006; In press: .
Li PP, Nakanishi A, Fontanes V, Kasamatsu H   Pairs of Vp1 cysteine residues essential for simian virus 40 infection Journal of Virology , 2005; 79: 3859-3864.
Li PP, Naknanishi A, Tran MA, Ishizu K, Kawano M, Phillips M, Handa H, Liddington RC, Kasamatsu H   Importance of Vp1 calcium-binding residues in assembly, cell entry, and nuclear entry of simian virus 40 Journal of Virology, 2003; 77: 7527-7538.
Nakanishi A, Shum D, Morioka H, Otsuka E and Kasamatsu H   Interaction of the Vp3 nuclear localization signal with the importin alpha2/beta heterodimer directs nuclear entry of infecting simian virus 40, Journal of Virology , 2002; 76: 9368-9377.
Li PP, Nakanishi A, Clark SW, Kasamatsu H   Formation of transitory intrachain and interchain disulfide bonds accompanies the folding and oligomerization of simian virus 40 Vp1 in the cytoplasm Proc. Natl. Acad. Sci. U.S.A. , 2002; 99: 1353-1358.
Li, P.P., Nakanishi, A., Shum, D., Sun, P.C.-K., Salazar, A.M., Fernandez, C.F., Chan, S.-W., and Kasamatsu, H.   Simian virus 40 Vp1 DNA binding domain is functionally separable from the overlapping nuclear localization signal and is required for effective virion formation and full viability, Journal of Virology, 2001; 75: 7321-7329.
Li PP, Nakanishi A, Tran MA, Salazar AM, Liddington RC, Kasamatsu H   Role of simian virus 40 Vp1 cysteines in virion infectivity Journal of Virology , 2000; 74: 11388-11393.
Nakanishi, A., Guan, L., Kane, R.R., Kasamatus, H., and Hawthorne, M.F.   Toward a cancer therapy with boron-rich oligomeric phosphate diesters that target the cell nucleus, Proc. Natl. Acad. Sci. U.S.A. , 1999; 96: 238-241.
Kasamatsu H and Nakanishi A   How do animal DNA viruses get to the nucleus? Annual Review of Microbiology, 1998; 52: 627-686.

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