Overview of Core
The W. M. Keck Proteomics Center contains all the equipment necessary for running 1-D and 2-D gels, transferring mini and mid-size gels, gel imaging, 1-D and 2-D gel analysis, spot cutting, in-gel digestion, 1-D and 2-D gel analysis, protein/peptide identification, mass spectrometry analysis, and data mining for a large variety of proteomics studies. In addition, we provide expert guidance in sample preparation for 1-D and 2-D gel electrophoresis and mass spectrometry, experiment design, gel staining including multiplex staining that compares total protein staining to phosphoprotein and glycoprotein staining, analysis of protein expression patterns, Western and lectin blots, in-gel tryptic digestion and operation of the various instruments. Researchers are encouraged to be trained in the technologies and to operate the equipment. The laboratory is a part of the UCLA Molecular Instrumentation Center (MIC), which is a campus wide facility open to the UCLA community. Further information can be obtained at the website below.
Fee schedule:
Name of Product/Service
User-run Service:
| Amersham TE62 Transfer Unit (holds up to 8 Criterion Blots) |
$25.00 / run |
| Bio-Rad Protean Isoelectric Focusing Cell (holds up to 12 strips)1 |
$30.00 / run |
| Bio-Rad Criterion Electrophoresis Cell (holds up to 2 gels)2 |
$15.00 / run |
| Bio-Rad Criterion Dodeca Electrophoresis Cell (holds up to 12 gels)2 |
$30.00 / run |
| Bio-Rad Protean II xi Electrophoresis Cell (holds up to 2 gels)2 |
$20.00 / run |
| Bio-Rad Protean Plus Dodeca Electrophoresis Cell (holds up to 12 gels)3 |
$75.00 / run |
| Bio-Rad Mini-Protean Tetra Cell (holds up to 4 gels)2 |
$15.00 / run |
| Bio-Rad Mini Trans Blot Transfer Cell (holds up to 2 gels) |
$15.00 / run |
| Invitrogen XCell SureLock Mini-Cell (holds up to 2 gels) |
$15.00 / run |
| Bio-Rad Fx Fluorescence Imager |
$20.00 / hour |
| Bio-Rad GS-800 Densitometer |
$20.00 / hour |
| DIGELAB ProPic II Spot Cutter4 |
$40.00 / hour |
| User-Run Mass Spectra (day rate |
$20.00 / hour |
| User-Run Mass Spectra (night rate) |
$10.00 / hour |
| Nonlinear Dynamics Progenesis SameSpots 2-D Gel Analysis Software |
$50.00 / month |
| Nonlinear Dynamics Progenesis SameSpots 2-D Gel Analysis Software - unlimited access |
$500.00 / year |
| Access to the Proteomics Bioinformatics Resources |
$50.00 / month |
| Access to the Proteomics Bioinformatics Resources - unlimited access |
$500.00 / year |
Staff Service:
| 1-D Gel Electrophoresis5 | $80/gel |
| SYPRO Ruby Staining & Imaging of a 1-D Gel |
$25/gel |
| NanoLC-MS/MS-Protein Identification from Gel Bands/Spots of Simple Mixtures6,7 |
1 - 6 samples |
$150.00 / sample |
|
7 -12 samples |
$140.00 / sample |
|
13 + samples |
$130.00 / sample |
| NanoLC-MS/MS-Protein Identification from 1-D Gel Bands of Complex Mixtures6,7 |
$20.00 / sample |
| NanoLC-MS/MS-Protein Identification from 2-D Gel Spots previously digested with trypsin |
$80.00 / sample |
| Staff-Run NanoLC-MS/MS run8 |
Instrument time |
$20.00 / hour |
|
Set up fee |
$60.00 / hour |
| Creating a database | $30.00 / each |
| Staff-Run Data Analysis | $60.00 / hour |
| Staff-Run MS, Sample Preparation, 2-D Gels, etc., excluding reagent costs |
$60.00 / hour |
| Training | $60.00 / hour |
| Supplies9 | cost +20% |
Fixed-Cost Training Sessions:
| Training to learn how to run 1-D gels |
$60 |
| Training to learn how to run 2-D gels10 |
$250 |
| Training to learn how to do a Transfer/Western Blot |
$60 |
| Training to use the Spotcutter |
$60 |
| Training to use the Imagers |
Free |
1Maximum charge per run is $30 per 24 hour period.
2 Rate includes the Running Buffer.
3 Proteomics staff will supply reagents for you to make your own Running Buffer.
4 Rate includes the 96-well plate and the sealing tape to cover the 96-well plate.
5 Up to 8 samples. Does not include cost of imaging and staining.
6Includes in-gel trypsin digestion, database searching, and consultation about the results.
7 The proteomics staff-scientist will determine whether the mixture is simple or complex.
Simple mixture example: eluents from a pull-down assay and co-immunoprecipitation
Complex mixture example: cell lysate
8 Sample preparation and data analysis done by user. Instrument time includes a blank run between each sample and quality control runs every 4 -5 samples.
Set up fee covers actual staff time on setting up the instrument and optimization of experimental parameters for your sample(s).
9 Minimum $1.00 charge for supplies that cost less than $1.00.
10 Includes consultation to work out optimum experimental conditions, one-on-one training to run and image 2-D gels (Maximum 2 people per session.)
* Location:
Laboratory: Molecular Sciences Building - Room 1424
* Personnel:
MIC Director
Jane Strouse, Ph.D. - Molecular Sciences Building, Room 1410
Proteomics Staff
Melissa Sondej, Ph.D. - Molecular Sciences Building, Room 1430
Rudy Alvarado, B.S. -Molecular Sciences Building, Room 1430 (enter through Room 1424)
