The ES Cell/Transgenic Mice Shared Resource provides a multi-tiered service to accommodate individual investigators' needs in the production of transgenic mice, using pronuclear injection; targeted genetic alterations in embryonic stem (ES) cells and murine strains derived from ES cells with targeted mutations. The mission of the ES Cell/Transgenic Mice Shared Resource is to provide these services with high standards of quality control in a cost-effective manner. The ES Cell/Transgenic Mice Shared Resource provides advice to investigators in the design and assembly of DNA constructs, on the availability of transgenic animal models and for evaluating and implementing new methodologies as they arise. In addition to providing consulting services, the ES Cell/Transgenic Mice Shared Resource provides materials (expression vectors, quality-assured ES cells, batch-tested sera and reagents) to investigators opting to undertake these projects in their own laboratories. The ES Cell/Transgenic Mice Shared Resource has two facilities: in addition to providing advice and reagents, the ES Cell Culture Facility will perform electroporations and select and maintain targeted ES cell lines for individual investigators; and the Transgenic/KO Injection Facility of the shared resource performs pronuclear injections of DNA and blastocyst injections of targeted ES cells, and maintains transgenic animal lines in their initial stages.
Embryonic Stem Cells:
For a standard construct, charges are $3,500, and for constructs requiring two rounds of selection (such as "hit-and run" constructs for subtle mutations), charges are $6,500. The ES Cell Facility expands 6 colonies for blastocyst injection as a part of the recharge service. The Shared Facility charges $350 for expansion of additional ES cell lines or cell lines not prepared in the ES Cell Facility. The ES Cell Facility also provides germline-competent ES cells ($350/vial) and mycoplasma-free primary embryonic feeder cells ($250 for one vial of expandable MEF).
Transgenic Mouse Facility:
The ES Cell/Transgenic Mice Shared Resource is administered by the Department of Molecular and Medical Pharmacology. Dr. Hong Wu will be assisted by the members of the Advisory Committee. The Advisory Committee meets on an ad hoc basis to discuss operation and pricing of services provided by the shared resource. This group also determines usage priorities, should demand exceed capacity. Services to be provided are scheduled by the director of the facility. The Embryonic Stem Cell Facility service begins with an initial consultation. The director or technician reviews vector construction strategy and screening protocols. Once a targeting vector has been constructed by the investigator, a request form is filled out that describes the vector and the project. The Transgenic/Knockout Injection Facility must receive a project request before services can be scheduled. This policy ensures that animal models generated by the shared resource are appropriately documented, and that investigators and the shared resource are in compliance with UCLA Animal Research Committee guidelines.
Center for Health Sciences (CHS) Dept. of Molecular & Medical Pharmacology
UCLA School of Medicine
650 S Charles Young Dr.
Los Angeles, CA, 90095
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